Anti-LMO2 Antibody Picoband™

Product Name

Anti-LMO2 Antibody Picoband™

View all LMO2 Antibodies

SKU/Catalog Number

A03502-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-LMO2 Antibody Picoband™ catalog # A03502-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LMO2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03502-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LMO2, which shares 97% amino acid (aa) sequence identity with both mouse and rat LMO2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03502-1 is reactive to LMO2 in Human, Mouse, Rat

Applications

A03502-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

Background of LMO2

LIM domain only 2 (rhombotin-like 1), also known as LMO2, RBTNL1, RBTN2, RHOM2, LIM Domain Only Protein 2, TTG2, and T-Cell Translocation Protein 2, is a protein which in humans is encoded by the LMO2 gene. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot,0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of LMO2 using anti-LMO2 antibody (A03502-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human SMMC-7721 whole cell lysates,
Lane 3: human A375 whole cell lysates,
Lane 4: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO2 antigen affinity purified polyclonal antibody (Catalog # A03502-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO2 at approximately 23KD. The expected band size for LMO2 is at 18KD.

Click image to see more details

Figure 2. Western blot analysis of LMO2 using anti-LMO2 antibody (A03502-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat C6 whole cell lysates,
Lane 2: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO2 antigen affinity purified polyclonal antibody (Catalog # A03502-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMO2 at approximately 23KD. The expected band size for LMO2 is at 18KD.

Gene/Protein Information For LMO2 (Source: Uniprot.org, NCBI)

Alternative Names

Cysteine-rich protein TTG-2; LIM domain only 2 (rhombotin-like 1); LIM domain only protein 2; LMO2; LMO-2; RBTN2; RBTN2rhombotin-2; RBTNL1; RBTNL1rhombotin-like 1; RHOM2; RHOM2T-cell translocation protein 2; T-cell translocation gene 2; TTG2; TTG2rhombotin 2 LMO2 LMO-2, RBTN2, RBTNL1, RHOM2, TTG2 LIM domain only 2 rhombotin-2|LIM domain only protein 2|T-cell translocation gene 2|T-cell translocation protein 2|cysteine-rich protein TTG-2|rhombotin-like 1

For more info on LMO2, check out the LMO2 Infographic

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In this infographic, you will see the following information for LMO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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