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Product Info Summary
| SKU: | M00666 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TMPRSS2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00666
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TMPRSS2 Rabbit Monoclonal Antibody catalog # M00666. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMPRSS2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00666)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
23T34
Isotype
IgG
Immunogen
A synthesized peptide derived from human TMPRSS2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00666 is reactive to TMPRSS2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated molecular weight
65894 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00666 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:100-1:500
FC 1:60
Positive Control
FCM: U2OS cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TMPRSS2 using anti-TMPRSS2 antibody (M00666).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human LNCAP whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMPRSS2 antigen affinity purified monoclonal antibody (Catalog # M00666) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMPRSS2 at approximately 54 kDa. The expected band size for TMPRSS2 is at 54 kDa.
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Figure 2. Flow Cytometry analysis of U2OS cells using anti-TMPRSS2 antibody (M00666).
Overlay histogram showing U2OS cells stained with M00666 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TMPRSS2 Antibody (M00666) at 1:60 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) at 1:60 was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:60 used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For TMPRSS2 (Source: Uniprot.org, NCBI)
Gene Name
TMPRSS2
Full Name
Transmembrane protease serine 2
Weight
65894 MW
Superfamily
peptidase S1 family
Alternative Names
Matrix metalloproteinase-14;MMP-14;3.4.24.80;MMP-X1;Membrane-type matrix metalloproteinase 1;MT-MMP 1;MTMMP1;Membrane-type-1 matrix metalloproteinase;MT1-MMP;MT1MMP;MMP14; TMPRSS2 PRSS10 transmembrane serine protease 2 transmembrane protease serine 2|epitheliasin|serine protease 10|transmembrane protease, serine 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMPRSS2, check out the TMPRSS2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMPRSS2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMPRSS2 Rabbit Monoclonal Antibody (M00666)
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