Click image to see more details
Product Info Summary
| SKU: | A04919 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Nephrocystin-4 NPHP4 Antibody
SKU/Catalog Number
A04919
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Nephrocystin-4 NPHP4 Antibody (Catalog # A04919). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nephrocystin-4 NPHP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04919)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human NPHP4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04919 is reactive to NPHP4 in Human
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
157598 MW
Background of Nphp4
Involved in the organization of apical junctions in kidney cells together with NPHP1 and RPGRIP1L/NPHP8 By similarity. Does not seem to be strictly required for ciliogenesis By similarity.
Mollet G., Nat. Genet. 32:300-305(2002).
Mollet G., Nat. Genet. 32:459-459(2002).
Otto E., Am. J. Hum. Genet. 71:1161-1167(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04919 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using NPHP4 antibody A04919.
Protein Target Info & Infographic
Gene/Protein Information For NPHP4 (Source: Uniprot.org, NCBI)
Gene Name
NPHP4
Full Name
Nephrocystin-4
Weight
157598 MW
Superfamily
NPHP4 family
Alternative Names
Nephrocystin-4;Nephroretinin;NPHP4;KIAA0673; Nphp4|4930564O18Rik, nmf19, nmf192|nephronophthisis 4 (juvenile) homolog (human)|nephrocystin-4|nephroretinin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPHP4, check out the NPHP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPHP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nephrocystin-4 NPHP4 Antibody (A04919)
Hello CJ!
No publications found for A04919
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Nephrocystin-4 NPHP4 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Nephrocystin-4 NPHP4 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-Nephrocystin-4 NPHP4 Antibody
Question
Is there a BSA free version of anti-NPHP4 antibody A04919 available?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-NPHP4 antibody A04919 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-14
Question
My question regarding product A04919, anti-NPHP4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04919 anti-NPHP4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-27
Question
I see that the anti-NPHP4 antibody A04919 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-30
Question
Will anti-NPHP4 antibody A04919 work for IHC with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to the expression profile of cervix carcinoma erythroleukemia, NPHP4 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-NPHP4 antibody A04919 will work for IHC with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-08-30
Question
Is this A04919 anti-NPHP4 antibody reactive to the isotypes of NPHP4?
Verified Customer
Verified customer
Asked: 2019-04-24
Answer
The immunogen of A04919 anti-NPHP4 antibody is Synthesized peptide derived from internal of human NPHP4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-24
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-NPHP4 antibody A04919. Let me know if you need anything else.
D. Edwards
Verified customer
Asked: 2018-10-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-02
Question
Is a blocking peptide available for product anti-NPHP4 antibody (A04919)?
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
We do provide the blocking peptide for product anti-NPHP4 antibody (A04919). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-14
Question
Will A04919 anti-NPHP4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
It shows on the product datasheet, A04919 anti-NPHP4 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-29
Question
I was wanting to use your anti-NPHP4 antibody for IHC for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
As indicated on the product datasheet, A04919 anti-NPHP4 antibody has been tested for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-26
Question
Will anti-NPHP4 antibody A04919 work on bovine IHC with testis?
C. Krishna
Verified customer
Asked: 2016-05-03
Answer
Our lab technicians have not validated anti-NPHP4 antibody A04919 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-NPHP4 antibody A04919 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine testis in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-03
Question
Our lab want to know about to test anti-NPHP4 antibody A04919 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-NPHP4 antibody A04919 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Krishna
Verified customer
Asked: 2015-10-15
Answer
The products we sell, including anti-NPHP4 antibody A04919, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-10-15
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-NPHP4 antibody A04919. Please let me know if you require anything else.
N. Singh
Verified customer
Asked: 2014-08-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-25
Question
We are currently using anti-NPHP4 antibody A04919 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
B. Miller
Verified customer
Asked: 2014-03-05
Answer
The anti-NPHP4 antibody (A04919) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-05
