Product Info Summary
SKU: | A13764-3 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-TMEM30B Antibody Picoband®
SKU/Catalog Number
A13764-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TMEM30B Antibody Picoband® catalog # A13764-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TMEM30B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13764-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TMEM30B recombinant protein (Position: M1-K300).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A13764-3 is reactive to TMEM30B in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
64099 MW
Background of TMEM30B
Enables aminophospholipid flippase activity. Involved in aminophospholipid transport and positive regulation of protein exit from endoplasmic reticulum. Predicted to be integral component of membrane. Predicted to be active in Golgi apparatus; endoplasmic reticulum; and plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13764-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human MCF-7 whole cell, human 293T whole cell, mouse RAW2647 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TMEM30B using anti-TMEM30B antibody (A13764-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM30B antigen affinity purified polyclonal antibody (Catalog # A13764-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM30B at approximately 39 kDa. The expected band size for TMEM30B is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TMEM30B (Source: Uniprot.org, NCBI)
Gene Name
TMEM30B
Full Name
Cell cycle control protein 50B
Weight
64099 MW
Superfamily
CDC50/LEM3 family
Alternative Names
RNA-binding protein 47;RNA-binding motif protein 47;RBM47; TMEM30B CDC50B transmembrane protein 30B cell cycle control protein 50B|P4-ATPase flippase complex beta subunit TMEM30B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMEM30B, check out the TMEM30B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMEM30B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMEM30B Antibody Picoband® (A13764-3)
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