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Product Info Summary
| SKU: | A14889 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TMEM240 Antibody Picoband®
SKU/Catalog Number
A14889
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TMEM240 Antibody Picoband® catalog # A14889. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMEM240 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14889)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TMEM240 recombinant protein (Position: M1-L173). Human TMEM240 shares 97.7% amino acid (aa) sequence identity with mouse TMEM240.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A14889 is reactive to TMEM240 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
19908 MW
Background of TMEM240
TMEM240 is a small, highly conserved transmembrane protein expressed in brain. The TMEM240 gene maps to chromosome 1p36.33. This gene encodes a transmembrane-domain containing protein found in the brain and cerebellum. Mutations in this gene result in spinocerebellar ataxia 21.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14889 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, NIH3T3 whole Cell, COLO320 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TMEM240 using anti-TMEM240 antibody (A14889).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: NIH3T3 whole Cell lysates,
Lane 3: COLO320 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM240 antigen affinity purified polyclonal antibody (Catalog # A14889) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM240 at approximately 19KD. The expected band size for TMEM240 is at 19KD.
Protein Target Info & Infographic
Gene/Protein Information For TMEM240 (Source: Uniprot.org, NCBI)
Gene Name
TMEM240
Full Name
Transmembrane protein 240
Weight
19908 MW
Superfamily
TMEM240 family
Alternative Names
Transmembrane protein 240;TMEM240;C1orf70; TMEM240 C1orf70, SCA21 transmembrane protein 240 transmembrane protein 240|transmembrane protein C1orf70
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMEM240, check out the TMEM240 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMEM240: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMEM240 Antibody Picoband® (A14889)
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No publications found for A14889
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-TMEM240 Antibody Picoband®
Question
Do you have a BSA free version of anti-TMEM240 antibody A14889 available?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TMEM240 antibody A14889 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-17
Question
My question regards to test anti-TMEM240 antibody A14889 on rat right hemisphere of cerebellum for research purposes, then I may be interested in using anti-TMEM240 antibody A14889 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Parker
Verified customer
Asked: 2018-09-24
Answer
The products we sell, including anti-TMEM240 antibody A14889, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-24
Question
Would anti-TMEM240 antibody A14889 work for WB with right hemisphere of cerebellum?
G. Jones
Verified customer
Asked: 2017-09-06
Answer
According to the expression profile of right hemisphere of cerebellum, TMEM240 is highly expressed in right hemisphere of cerebellum. So, it is likely that anti-TMEM240 antibody A14889 will work for WB with right hemisphere of cerebellum.
Boster Scientific Support
Answered: 2017-09-06
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right hemisphere of cerebellum using anti-TMEM240 antibody A14889. Let me know if you need anything else.
D. Rodriguez
Verified customer
Asked: 2016-05-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-11
Question
I have a question about product A14889, anti-TMEM240 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Jones
Verified customer
Asked: 2016-02-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14889 anti-TMEM240 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-02-11
Question
We are currently using anti-TMEM240 antibody A14889 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
S. Krishna
Verified customer
Asked: 2015-02-03
Answer
The anti-TMEM240 antibody (A14889) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-02-03
