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Product Info Summary
| SKU: | A08035 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-TM16G ANO7 Antibody
SKU/Catalog Number
A08035
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TM16G ANO7 Antibody (Catalog # A08035). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TM16G ANO7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08035)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human TM16G.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08035 is reactive to ANO7 in Human
Reconstitution
Calculated molecular weight
105532 MW
Background of ANO7
Does not exhibit calcium-activated chloride channel (CaCC) activity. May play a role in cell-cell interactions.
Bera T.K., Proc. Natl. Acad. Sci. U.S.A. 101:3059-3064(2004).
Walker M.G., Genome Res. 9:1198-1203(1999).
Katoh M., Int. J. Mol. Med. 14:759-764(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08035 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using TM16G antibody A08035.
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Western blot analysis of extracts from LOVO cells, using TM16G antiobdy A08035.
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Immunofluorescence analysis of HepG2 cells, using TM16G antibody A08035.
Protein Target Info & Infographic
Gene/Protein Information For ANO7 (Source: Uniprot.org, NCBI)
Gene Name
ANO7
Full Name
Anoctamin-7
Weight
105532 MW
Superfamily
anoctamin family
Alternative Names
Anoctamin-7;Dresden transmembrane protein of the prostate;D-TMPP;IPCA-5;New gene expressed in prostate;Prostate cancer-associated protein 5;Transmembrane protein 16G;ANO7;NGEP, PCANAP5, TMEM16G; ANO7 D-TMPP, DTMPP, IPCA-5, IPCA5, NGEP, PCANAP5, PCANAP5L, TMEM16G anoctamin 7 anoctamin-7|Dresden-transmembrane protein of the prostate|prostate cancer associated protein 5|transmembrane protein 16G
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANO7, check out the ANO7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANO7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TM16G ANO7 Antibody (A08035)
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No publications found for A08035
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TM16G ANO7 Antibody
Question
Would anti-TM16G antibody A08035 work on canine IF with prostate gland?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Our lab technicians have not tested anti-TM16G antibody A08035 on canine. You can run a BLAST between canine and the immunogen sequence of anti-TM16G antibody A08035 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine prostate gland in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-21
Question
Does A08035 anti-TM16G antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Li
Verified customer
Asked: 2018-09-11
Answer
As indicated on the product datasheet, A08035 anti-TM16G antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-11
Question
We are currently using anti-TM16G antibody A08035 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
The anti-TM16G antibody (A08035) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-02
Question
Is this A08035 anti-TM16G antibody reactive to the isotypes of ANO7?
G. Johnson
Verified customer
Asked: 2016-01-15
Answer
The immunogen of A08035 anti-TM16G antibody is Synthesized peptide derived from C-terminal of human TM16G. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-01-15
