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Product Info Summary
| SKU: | A01541-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Tlr8 Antibody Picoband®
SKU/Catalog Number
A01541-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tlr8 Antibody Picoband® catalog # A01541-4. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Tlr8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01541-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Tlr8, which shares 76.5% amino acid (aa) sequence identity with human TLR8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01541-4 is reactive to Tlr8 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
119 kDa
Calculated molecular weight
119.828kDa
Background of TLR8
TLR8 (Toll-like receptor 8) is a protein that in humans is encoded by the TLR8 gene. TLR8 has also been designated as CD288 (cluster of differentiation 288).The TLR8 gene is mapped to Xp22.3-p22.2 by Chuang and Ulevitch (2000) and Du et al. (2000). The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. TLR8 recognises G-rich oligonucleotides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01541-4 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Positive Control
WB: mouse Ana-1 whole cell
IHC: mouse lung tissue, rat lung tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Tlr8 using anti-Tlr8 antibody (A01541-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr8 antigen affinity purified polyclonal antibody (Catalog # A01541-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr8 at approximately 119 kDa. The expected band size for Tlr8 is at 119 kDa.
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Figure 2. IHC analysis of Tlr8 using anti-Tlr8 antibody (A01541-4).
Tlr8 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tlr8 Antibody (A01541-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Tlr8 using anti-Tlr8 antibody (A01541-4).
Tlr8 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tlr8 Antibody (A01541-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Tlr8 (Source: Uniprot.org, NCBI)
Gene Name
Tlr8
Full Name
Toll-like receptor 8
Weight
119.828kDa
Superfamily
Toll-like receptor family
Alternative Names
Nuclear factor 1 B-type; NF1-B; Nuclear factor 1/B; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/B; NF-I/B; NFI-B; TGGCA-binding protein; NFIB TLR8 CD288 toll like receptor 8 toll-like receptor 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tlr8, check out the Tlr8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tlr8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tlr8 Antibody Picoband® (A01541-4)
Hello CJ!
A01541-4 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of lipopolysaccharide‐mediated rat alveolar macrophage activation in vitro by antiflammin‐1
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