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Product Info Summary
| SKU: | M01541 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TLR8 Mouse Monoclonal Antibody [Clone ID: OTI4F3]
SKU/Catalog Number
M01541
Size
100 µl
Description
Boster Bio TLR8 mouse monoclonal antibody,clone OTI4F3. Catalog# M01541. Tested in IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLR8 Mouse Monoclonal Antibody [Clone ID: OTI4F3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01541)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI4F3
Isotype
IgG2a
Immunogen
Human recombinant protein fragment corresponding to amino acids 32-153 of human TLR8 (NP_619542) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01541 is reactive to TLR8 in Human
Reconstitution
Observed Molecular Weight
119.6 kDa
Calculated molecular weight
119.828kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01541 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-TLR8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human spleen tissue within the normal limits using anti-TLR8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLR8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TLR8 (1:2000).
Protein Target Info & Infographic
Gene/Protein Information For TLR8 (Source: Uniprot.org, NCBI)
Gene Name
TLR8
Full Name
Toll-like receptor 8
Weight
119.828kDa
Superfamily
Toll-like receptor family
Alternative Names
CD288 antigen; CD288; MGC119599; MGC119600; TLR8; toll-like receptor 8 TLR8 CD288 toll like receptor 8 toll-like receptor 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR8, check out the TLR8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TLR8 Mouse Monoclonal Antibody [Clone ID: OTI4F3] (M01541)
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No publications found for M01541
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Customer Q&As
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15 Customer Q&As for Anti-TLR8 Mouse Monoclonal Antibody [Clone ID: OTI4F3]
Question
We have been able to see staining in mouse blood. What should we do? Is anti-Toll-like Receptor 8 Monoclonal antibody (44C143) supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Based on literature blood does express TLR8. Based on Uniprot.org, TLR8 is expressed in blood, placenta, liver, among other tissues. Regarding which tissues have TLR8 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 11022119, 11022120, 18810425
Boster Scientific Support
Answered: 2020-04-24
Question
Will anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
According to the expression profile of placenta, TLR8 is highly expressed in placenta. So, it is likely that anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541 will work for WB with placenta.
Boster Scientific Support
Answered: 2020-04-17
Question
Do you have a BSA free version of anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541 available?
K. Jones
Verified customer
Asked: 2020-04-10
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-10
Question
My team were content with the WB result of your anti-Toll-like Receptor 8 Monoclonal antibody (44C143) . However we have seen positive staining in placenta membrane using this antibody. Is that expected? Could you tell me where is TLR8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
According to literature, placenta does express TLR8. Generally TLR8 expresses in membrane. Regarding which tissues have TLR8 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Placenta, Pubmed ID: 11022119, 11022120, 18810425
Boster Scientific Support
Answered: 2020-04-03
Question
I was wanting to use your anti-Toll-like Receptor 8 Monoclonal antibody (44C143) for WB for mouse placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
You can see on the product datasheet, M01541 anti-Toll-like Receptor 8 Monoclonal antibody (44C143) has been tested for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-18
Question
My question regards to test anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541 on mouse placenta for research purposes, then I may be interested in using anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The products we sell, including anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-30
Question
We are currently using anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The anti-Toll-like Receptor 8 Monoclonal antibody (44C143) (M01541) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-11
Question
Is a blocking peptide available for product anti-Toll-like Receptor 8 Monoclonal antibody (44C143) (M01541)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We do provide the blocking peptide for product anti-Toll-like Receptor 8 Monoclonal antibody (44C143) (M01541). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-27
Question
Will M01541 anti-Toll-like Receptor 8 Monoclonal antibody (44C143) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
As indicated on the product datasheet, M01541 anti-Toll-like Receptor 8 Monoclonal antibody (44C143) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-26
Question
We ordered your anti-Toll-like Receptor 8 Monoclonal antibody (44C143) for WB on blood a few years ago. I am using mouse, and We are going to use the antibody for IHC next. We need examining blood as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
I have checked the website and datasheets of our anti-Toll-like Receptor 8 Monoclonal antibody (44C143) and it appears that M01541 has been validated on mouse in both WB and IHC. Thus M01541 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-11-06
Question
I see that the anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-04
Question
Is this M01541 anti-Toll-like Receptor 8 Monoclonal antibody (44C143) reactive to the isotypes of TLR8?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
The immunogen of M01541 anti-Toll-like Receptor 8 Monoclonal antibody (44C143) is Synthetic peptide corresponding to aa 750-850 of the cytoplasmic portion of human TLR8 (Toll-like receptor 8) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-05
Question
I have attached the WB image, lot number and protocol we used for placenta using anti-Toll-like Receptor 8 Monoclonal antibody (44C143) M01541. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-15
Question
I have a question about product M01541, anti-Toll-like Receptor 8 Monoclonal antibody (44C143) . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Patel
Verified customer
Asked: 2017-08-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01541 anti-Toll-like Receptor 8 Monoclonal antibody (44C143) , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-02
