Click image to see more details
Product Info Summary
| SKU: | PA1733 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Toll-like receptor 7 TLR7 Antibody Picoband®
SKU/Catalog Number
PA1733
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Toll-like receptor 7 TLR7 Antibody catalog # PA1733. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Toll-like receptor 7 TLR7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1733)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1733 is reactive to TLR7 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
120922 MW
Background of TLR7
TLR7 (Toll-like receptor 7) is protein that in humans is encoded by the TLR7 gene. Orthologs are found in mammals and birds. TLR7 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1733 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Spleen Tissue, Rat Liver Tissue, U87 Cell, A549 Cell,
IHC: Human Placenta Tissue, Rat Spleen Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-TLR7 antibody, PA1733, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: A549 Cell Lysate
Click image to see more details
Anti-TLR7 antibody, PA1733, IHC(P)
IHC(P): Human Placenta Tissue
Click image to see more details
Anti-TLR7 antibody, PA1733, IHC(P)
IHC(P): Rat Spleen Tissue
Click image to see more details
Protein Target Info & Infographic
Gene/Protein Information For TLR7 (Source: Uniprot.org, NCBI)
Gene Name
TLR7
Full Name
Toll-like receptor 7
Weight
120922 MW
Superfamily
Toll-like receptor family
Alternative Names
Toll-like receptor 7;TLR7;UNQ248/PRO285; TLR7 IMD74-like, TLR7 toll like receptor 7 toll-like receptor 7|toll-like receptor 7-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR7, check out the TLR7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Toll-like receptor 7 TLR7 Antibody Picoband® (PA1733)
Hello CJ!
No publications found for PA1733
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Toll-like receptor 7 TLR7 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Toll-like receptor 7 TLR7 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
17 Customer Q&As for Anti-Toll-like receptor 7 TLR7 Antibody Picoband®
Question
Would anti-TLR7 antibody PA1733 work for WB with testis?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
According to the expression profile of testis, TLR7 is highly expressed in testis. So, it is likely that anti-TLR7 antibody PA1733 will work for WB with testis.
Boster Scientific Support
Answered: 2020-03-30
Question
Is this PA1733 anti-TLR7 antibody reactive to the isotypes of TLR7?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The immunogen of PA1733 anti-TLR7 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7(1020-1038aa QAHPYFWQCLKNALATDNH), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-27
Question
I have a question about product PA1733, anti-TLR7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Carter
Verified customer
Asked: 2019-12-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1733 anti-TLR7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-02
Question
We want using your anti-TLR7 antibody for microglial cell activation studies. Has this antibody been tested with western blotting on spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
Thanks for your inquiry. This PA1733 anti-TLR7 antibody is tested on rat liver tissue, spleen tissue, tissue lysate, u87 cell lysate, a549 cell lysate, human placenta tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-11
Question
I was wanting to use your anti-TLR7 antibody for WB for mouse testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
As indicated on the product datasheet, PA1733 anti-TLR7 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-14
Question
I see that the anti-TLR7 antibody PA1733 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-10
Question
We are currently using anti-TLR7 antibody PA1733 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The anti-TLR7 antibody (PA1733) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-15
Question
Would PA1733 anti-TLR7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
As indicated on the product datasheet, PA1733 anti-TLR7 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-29
Question
Will anti-TLR7 antibody PA1733 work on zebrafish IHC with testis?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
Our lab technicians have not validated anti-TLR7 antibody PA1733 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TLR7 antibody PA1733 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish testis in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-04
Question
We have seen staining in mouse testis. Do you have any suggestions? Is anti-TLR7 antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
According to literature testis does express TLR7. According to Uniprot.org, TLR7 is expressed in leukocyte, placenta, testis, among other tissues. Regarding which tissues have TLR7 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 11022119, 11022120, 19924287
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-TLR7 antibody PA1733. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-20
Question
See below the WB image, lot number and protocol we used for testis using anti-TLR7 antibody PA1733. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-16
Question
Is a blocking peptide available for product anti-TLR7 antibody (PA1733)?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
We do provide the blocking peptide for product anti-TLR7 antibody (PA1733). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-11
Question
My team were content with the WB result of your anti-TLR7 antibody. However we have observed positive staining in testis endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is TLR7 supposed to be expressed?
F. Wu
Verified customer
Asked: 2017-03-07
Answer
From what I have seen in literature, testis does express TLR7. Generally TLR7 expresses in endoplasmic reticulum membrane. Regarding which tissues have TLR7 expression, here are a few articles citing expression in various tissues:
Placenta, Pubmed ID: 11022119, 11022120, 19924287
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-03-07
Question
Is there a BSA free version of anti-TLR7 antibody PA1733 available?
L. Collins
Verified customer
Asked: 2015-09-15
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TLR7 antibody PA1733 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-09-15
Question
I am looking for to test anti-TLR7 antibody PA1733 on mouse testis for research purposes, then I may be interested in using anti-TLR7 antibody PA1733 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Walker
Verified customer
Asked: 2015-05-28
Answer
The products we sell, including anti-TLR7 antibody PA1733, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-05-28
Question
We ordered your anti-TLR7 antibody for WB on placenta in a previous project. I am using mouse, and We intend to use the antibody for IHC next. I am looking for examining placenta as well as testis in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
D. Wu
Verified customer
Asked: 2015-01-08
Answer
I have checked the website and datasheets of our anti-TLR7 antibody and it seems that PA1733 has been tested on mouse in both WB and IHC. Thus PA1733 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-01-08
