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Product Info Summary
| SKU: | EK0882 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Rat KIM1 ELISA Kit PicoKine®
View all TIM-1/KIM-1/HAVCR ELISA kits
SKU/Catalog Number
EK0882
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat KIM1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat KIM1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0882)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S18-V238
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0882 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0882 is reactive to Havcr1 in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK0882 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TIM-1/KIM-1/HAVCR
KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0882-CAP | Anti-Rat Havcr1 Pre-coated 96-well strip microplate | 1 |
| EK0882-ST | Rat Havcr1 Standard | 2 vials, 10 ng/tube |
| EK0882-DA | Rat Havcr1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.029 | 0.055 | 0.105 | 0.271 | 0.482 | 0.760 | 1.200 | 1.838 |
Data Example Images
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Rat KIM1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TIM-1/KIM-1/HAVCR, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Some articles we found to cite concentrations of TIM-1/KIM-1/HAVCR in samples: 22898836 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat KIM1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 87 | 272 | 938 | 86 | 293 | 1026 |
| Standard deviation | 3.82 | 20.67 | 59.09 | 3.95 | 23.14 | 84.13 |
| CV (%) | 4.4% | 7.6% | 7.9% | 4.6% | 7.9% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TIM-1/KIM-1/HAVCR in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 87 | 94 | 102 | 93 | 94 | 5.33 | 5.6% |
| Sample 2 | 272 | 238 | 277 | 253 | 260 | 15.54 | 5.9% |
| Sample 3 | 938 | 849 | 928 | 895 | 902 | 34.74 | 3.8% |
Protein Target Info & Infographic
Gene/Protein Information For Havcr1 (Source: Uniprot.Org, NCBI)
Gene Name
Havcr1
Full Name
Hepatitis A virus cellular receptor 1
Weight
33964 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Hepatitis A virus cellular receptor 1 homolog;HAVcr-1;Kidney injury molecule 1;KIM-1;T cell immunoglobulin and mucin domain-containing protein 1;TIMD-1;Havcr1;Kim1; HAVCR1 CD365, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1 hepatitis A virus cellular receptor 1 hepatitis A virus cellular receptor 1|T cell immunoglobin domain and mucin domain protein 1|T-cell immunoglobulin mucin family member 1|T-cell immunoglobulin mucin receptor 1|T-cell membrane protein 1|kidney injury molecule 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Havcr1, check out the Havcr1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Havcr1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat KIM1 ELISA Kit PicoKine® (EK0882)
Hello CJ!
EK0882 has been cited in 13 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Comparison of biomarkers in rat renal ischemia-reperfusion injury
Acute kidney injury during pregnancy leads to increased sFlt-1 and sEng and decreased renal T regulatory cells in pregnant rats with HELLP syndrome
Can remote ischemic preconditioning counteract the renal functional deterioration attributable to partial nephrectomy under warm ischemia? Results of an animal study
Exogenously administered adenosine attenuates renal damage in streptozotocin-induced diabetic rats
Nonalcoholic fatty liver disease alters microcystin-LR toxicokinetics and acute toxicity
Possible mechanisms for the renoprotective action of adipose-derived mesenchymal stem cells with CD44-targeted hyaluronic acid against renal ischemia
Clove (Syzygium aromaticum) and honey extracts significantly reduce inflammatory cytokines and liver function enzymes in experimental rats fed on carbon tetrachloride (CCl4)
Key role of organic cation transporter 2 for the nephrotoxicity effect of triptolide in rheumatoid arthritis
Protective effect of Salvianolic acid A on ischaemia‐reperfusion acute kidney injury in rats through protecting against peritubular capillary endothelium damages
Awadalla A,Hussein AM,Ali M,Barakat N,Hamam ET,Magar RW,Shokeir AA. Possible mechanisms for the renoprotective action of adipose-derived mesenchymal stem cells with CD44-targeted hyaluronic acid against renal ischemia. Life Sci.2021 Feb 17:119221.doi:10.1
Species: Rat
EK0882 usage in article: APP:ELISA, SAMPLE:URINE, DILUTION:NA
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Rat KIM1 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
E. Zhao
Verified customer
Asked: 2020-10-21
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-10-21
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Jones
Verified customer
Asked: 2020-05-29
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-05-29
Question
Q: Can TIM-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-01-05
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-01-05
Question
Q: how to proceed with the analysis of ELISA data? I have performed TIM-1 level in serum.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-10-03
Question
Does EK0882 work with mouse samples?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
EK0882 is not suitable for working on mouse samples?
Boster Scientific Support
Answered: 2019-08-07
Question
How would having twice the volume of ABC in standards while 50% volume of ABC in all other wells affect the end result using EK0882?
Verified customer
Asked: 2019-07-17
Answer
For the Rat KIM1 ELISA Kit PicoKine™ (EK0882), the following points may help you: 1. Know that the ABC amount is redundant, so that having twice the amount of ABC in standard wells will only affect the readings marginally, skewing the results higher. 2. Having 50% of ABC in sample wells could be problematic if the samples have high amounts of KIM1 in them. Whether that is the case, you will have to decide based on the specific models you are using. If the level of KIM1 is low, it is expected to only marginally affect the readings, skewing the results lower. If KIM1 is high, and binds to more capture antibodies than there is ABC (at only 50% amount) to fully cover, then it would skew the results a lot. This is unlikely to happen but please check to see if your model has a very high level of KIM1. 3. With the same amount analytes generating higher readings in standard wells and lower readings in sample wells, your conclusion of the amount of KIM1 in each sample will be skewed lower than their actual value. That means, the deduced level of KIM1 is lower than the actual level of KIM1. Due to the redundancy in the amount of ABC, the deduced value should be closer to the actual value than the 50%. The data is usable as guidance for further experiment designs but is likely not acceptable for publication. The above are our technical opinions. Please take in considerations of other circumstances that we are not aware of and make a comprehensive decision.
Boster Scientific Support
Answered: 2019-07-18
Question
Is EK0882 suitable for working on mouse kidney tissues?
Verified customer
Asked: 2019-05-17
Answer
The Rat KIM1 ELISA Kit PicoKine™ (EK0882) is not suitable for working on mouse samples. Our lab hasn't worked on tissue samples using the kit. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend to run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, you can proceed and work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-05-17
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis TIM-1 using the EK0882 Rat TIM-1 Picokine® ELISA Kit?
K. Zhao
Verified customer
Asked: 2019-04-11
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-04-11
Question
Q: can you suggest the dilution ratio of serum samples for detection of TIM-1 in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TIM-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-04-01
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-01-25
Question
Does the antibody in this kit cross react with the protein from mouse urine?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
We did not test the cross reactivity of antibody from this kit to mouse protein. Therefore, we cannot guarantee the result if you use it to mouse.
Boster Scientific Support
Answered: 2018-11-06
Question
Q: can I use citrate plasma as samples in Rat TIM-1 Picokine® ELISA Kit (Catalog # EK0882)?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TIM-1 causing degradation of its protein structure. TIM-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TIM-1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-12-26
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
A. Williams
Verified customer
Asked: 2015-09-05
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-09-05
