Click image to see more details
Product Info Summary
| SKU: | RP1036 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TIE2/TEK Antibody Picoband®
SKU/Catalog Number
RP1036
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TIE2/TEK Antibody catalog # RP1036. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIE2/TEK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1036)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TIE2 recombinant protein (Position: Q641-I830). Human TIE2 shares 91% amino acid (aa) sequence identity with mouse TIE2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1036 is reactive to TEK in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
125 kDa
Calculated molecular weight
125830 MW
Background of Tie-2
TIE2, also known as TEK tyrosine kinase, TIE2 gene is mapped to 9p21.2. This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1036 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-TIE2 Picoband antibody, RP1036-1.jpg
All lanes: Anti TIE2 (RP1036) at 0.5ug/ml
WB: Recombinant Human TIE2 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
Click image to see more details
Anti-TIE2 Picoband antibody, RP1036-2.jpg
All lanes: Anti TIE2 (RP1036) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 125KD
Observed bind size: 125KD
Protein Target Info & Infographic
Gene/Protein Information For TEK (Source: Uniprot.org, NCBI)
Gene Name
TEK
Full Name
Angiopoietin-1 receptor
Weight
125830 MW
Superfamily
protein kinase superfamily
Alternative Names
Angiopoietin-1 receptor;2.7.10.1;Endothelial tyrosine kinase;Tunica interna endothelial cell kinase;Tyrosine kinase with Ig and EGF homology domains-2;Tyrosine-protein kinase receptor TEK;Tyrosine-protein kinase receptor TIE-2;hTIE2;p140 TEK;CD202b;TEK;TIE2, VMCM, VMCM1; TEK CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1 TEK receptor tyrosine kinase angiopoietin-1 receptor|TEK tyrosine kinase, endothelial|endothelial tyrosine kinase|tunica interna endothelial cell kinase|tyrosine kinase with Ig and EGF homology domains-2|tyrosine-protein kinase receptor TEK|tyrosine-protein kinase receptor TIE-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TEK, check out the TEK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TEK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIE2/TEK Antibody Picoband® (RP1036)
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No publications found for RP1036
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-TIE2/TEK Antibody Picoband®
Question
We are currently using anti-TIE2/TEK antibody RP1036 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
The anti-TIE2/TEK antibody (RP1036) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-01
Question
See below the WB image, lot number and protocol we used for liver using anti-TIE2/TEK antibody RP1036. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
My question regarding product RP1036, anti-TIE2/TEK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1036 anti-TIE2/TEK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-06
Question
I was wanting to use your anti-TIE2/TEK antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
As indicated on the product datasheet, RP1036 anti-TIE2/TEK antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-02
Question
I would like to test anti-TIE2/TEK antibody RP1036 on human liver for research purposes, then I may be interested in using anti-TIE2/TEK antibody RP1036 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
The products we sell, including anti-TIE2/TEK antibody RP1036, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-23
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-TIE2/TEK antibody RP1036. Let me know if you need anything else.
M. Yang
Verified customer
Asked: 2016-02-02
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-02-02
