Anti-Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker) Monoclonal Antibody

Product Name

Anti-Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker) Monoclonal Antibody

View all PD-ECGF/Thymidine Phosphorylase Antibodies

SKU/Catalog Number

M06329

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker) Monoclonal Antibody (Catalog # M06329). Tested in WB, IP, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06329)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM322

Isotype

IgG1, kappa

Immunogen

Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M06329 is reactive to TYMP in Human, Mouse, Rat

Applications

M06329 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

49955 MW

Background of PD-ECGF/Thymidine Phosphorylase

Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5'-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
Immunoprecipitation (0.5-1ug/500ug protein lysate)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Placenta stained with Anti-Thymidine Phosphorylase / PD-ECGF Monoclonal Antibody (SPM322).

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Western Blot analysis of human spleen tissue lysate using Anti-Thymidine Phosphorylase / PD-ECGF Monoclonal Antibody (SPM322).

Gene/Protein Information For TYMP (Source: Uniprot.org, NCBI)

Alternative Names

dThdPase; EC 2.4.2; EC 2.4.2.4; ECGF; ECGF1; ECGF1TP; endothelial cell growth factor 1 (platelet-derived); Gliostatin; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; PD-ECGF; Platelet-derived endothelial cell growth factor; TdRPase; Thymidine Phosphorylase; TPase; TYMP TYMP ECGF, ECGF1, MEDPS1, MNGIE, MTDPS1, PDECGF, TP, hPD-ECGF thymidine phosphorylase thymidine phosphorylase|gliostatin|tdRPase

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In this infographic, you will see the following information for TYMP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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