Click image to see more details
Product Info Summary
| SKU: | A04619-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-THOC5/Fmip Antibody
SKU/Catalog Number
A04619-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-THOC5/Fmip Antibody (Catalog# A04619-1). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-THOC5/Fmip Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04619-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
7A7
Isotype
Rabbit IgG
Immunogen
A peptide derived from human THOC5. Immunogen sequence location: 561 - 610
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04619-1 is reactive to THOC5 in Human, Mouse, Rat
Applications
A04619-1 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
78508 MW
Background of THOC5
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using THOC5 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For THOC5 (Source: Uniprot.org, NCBI)
Gene Name
THOC5
Full Name
THO complex subunit 5 homolog
Weight
78508 MW
Superfamily
THOC5 family
Alternative Names
C22orf19; chromosome 22 open reading frame 19; Fmip; Fms-interacting protein; fSAP79hTREX90; Functional spliceosome-associated protein 79; KIAA0983; NF2/meningioma region protein pK1.3; PK1.3; Placental protein 39.2; PP39.2; THO complex 5; THO complex subunit 5 homolog THOC5 C22orf19, Fmip, PK1.3, fSAP79 THO complex 5 THO complex subunit 5 homolog|Fms-interacting protein|NF2/meningioma region protein pK1.3|PP39.2|functional spliceosome-associated protein 79|hTREX90|placental protein 39.2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on THOC5, check out the THOC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for THOC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-THOC5/Fmip Antibody (A04619-1)
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No publications found for A04619-1
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Customer Q&As
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13 Customer Q&As for Anti-THOC5/Fmip Antibody
Question
Do you have a BSA free version of anti-THOC5/Fmip antibody A04619-1 available?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-THOC5/Fmip antibody A04619-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-04
Question
Is this A04619-1 anti-THOC5/Fmip antibody reactive to the isotypes of THOC5?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The immunogen of A04619-1 anti-THOC5/Fmip antibody is A peptide derived from human THOC5. Immunogen sequence location: 561 - 610. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-30
Question
My question regarding product A04619-1, anti-THOC5/Fmip antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04619-1 anti-THOC5/Fmip antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-27
Question
We are currently using anti-THOC5/Fmip antibody A04619-1 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
The anti-THOC5/Fmip antibody (A04619-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-03
Question
Will anti-THOC5/Fmip antibody A04619-1 work on bovine IHC with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
Our lab technicians have not tested anti-THOC5/Fmip antibody A04619-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-THOC5/Fmip antibody A04619-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cervix carcinoma erythroleukemia in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-05
Question
Will anti-THOC5/Fmip antibody A04619-1 work for IHC with placenta?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
According to the expression profile of placenta, THOC5 is highly expressed in placenta. So, it is likely that anti-THOC5/Fmip antibody A04619-1 will work for IHC with placenta.
Boster Scientific Support
Answered: 2019-11-13
Question
Will A04619-1 anti-THOC5/Fmip antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
As indicated on the product datasheet, A04619-1 anti-THOC5/Fmip antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-10
Question
I have attached the WB image, lot number and protocol we used for placenta using anti-THOC5/Fmip antibody A04619-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-12
Question
I am interested in to test anti-THOC5/Fmip antibody A04619-1 on human placenta for research purposes, then I may be interested in using anti-THOC5/Fmip antibody A04619-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-16
Answer
The products we sell, including anti-THOC5/Fmip antibody A04619-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-16
Question
I was wanting to use your anti-THOC5/Fmip antibody for IHC for human placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human placenta identification?
M. Krishna
Verified customer
Asked: 2017-04-17
Answer
You can see on the product datasheet, A04619-1 anti-THOC5/Fmip antibody has been validated for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-17
Question
Is a blocking peptide available for product anti-THOC5/Fmip antibody (A04619-1)?
G. Carter
Verified customer
Asked: 2015-02-06
Answer
We do provide the blocking peptide for product anti-THOC5/Fmip antibody (A04619-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-02-06
Question
I see that the anti-THOC5/Fmip antibody A04619-1 works with IHC, what is the protocol used to produce the result images on the product page?
V. Carter
Verified customer
Asked: 2014-05-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-05-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-THOC5/Fmip antibody A04619-1. Let me know if you need anything else.
J. Edwards
Verified customer
Asked: 2013-08-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-16
