Anti-Thioredoxin 2 Monoclonal Antibody

Thioredoxin-2 antibody

Boster Bio Anti-Thioredoxin 2 Monoclonal Antibody catalog # M04586. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M04586
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Thioredoxin 2 Monoclonal Antibody

View all Thioredoxin-2 Antibodies

SKU/Catalog Number

M04586

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Thioredoxin 2 Monoclonal Antibody catalog # M04586. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Thioredoxin 2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04586)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEGD-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Thioredoxin 2 Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04586 is reactive to TXN2 in Human, Mouse, Rat

Applications

M04586 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

93231 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100

Validation Images & Assay Conditions

Gene/Protein Information For TXN2 (Source: Uniprot.org, NCBI)

Gene Name

TXN2

Full Name

Thioredoxin, mitochondrial

Weight

93231 MW

Superfamily

thioredoxin family

Alternative Names

mitochondrial thioredoxin; MTRX; MT-TRX; thioredoxin 2; thioredoxin, mitochondrial; Thioredoxin2; Thioredoxin-2; Trx2; TXN2 TXN2 COXPD29, MT-TRX, MTRX, TRX2, TXN thioredoxin 2 thioredoxin, mitochondrial|mitochondrial thioredoxin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TXN2, check out the TXN2 Infographic

TXN2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TXN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Thioredoxin 2 Monoclonal Antibody

Question

Will M04586 anti-Thioredoxin 2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

It shows on the product datasheet, M04586 anti-Thioredoxin 2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-08

Question

We want to test anti-Thioredoxin 2 Monoclonal antibody M04586 on rat liver for research purposes, then I may be interested in using anti-Thioredoxin 2 Monoclonal antibody M04586 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

The products we sell, including anti-Thioredoxin 2 Monoclonal antibody M04586, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-09-18

Question

I was wanting to use your anti-Thioredoxin 2 Monoclonal antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?

S. Mitchell

Verified customer

Asked: 2016-12-08

Answer

It shows on the product datasheet, M04586 anti-Thioredoxin 2 Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-12-08

Order DetailsPrice
M04586

100μL

$370
M04586-30ul

30μL sample

$99
M04586-carrier-free-1ml

Carrier Free

$2590

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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