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Product Info Summary
| SKU: | M04586 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Thioredoxin 2 Monoclonal Antibody
View all Thioredoxin-2 Antibodies
SKU/Catalog Number
M04586
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Thioredoxin 2 Monoclonal Antibody catalog # M04586. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Thioredoxin 2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04586)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEGD-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Thioredoxin 2 Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04586 is reactive to TXN2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
93231 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04586 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
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Western blot analysis of Thioredoxin 2 expression in Raji cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For TXN2 (Source: Uniprot.org, NCBI)
Gene Name
TXN2
Full Name
Thioredoxin, mitochondrial
Weight
93231 MW
Superfamily
thioredoxin family
Alternative Names
Caldesmon;CDM;CALD1;CAD, CDM; TXN2 COXPD29, MT-TRX, MTRX, TRX2, TXN thioredoxin 2 thioredoxin, mitochondrial|mitochondrial thioredoxin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TXN2, check out the TXN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TXN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thioredoxin 2 Monoclonal Antibody (M04586)
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No publications found for M04586
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Customer Q&As
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3 Customer Q&As for Anti-Thioredoxin 2 Monoclonal Antibody
Question
Will M04586 anti-Thioredoxin 2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
It shows on the product datasheet, M04586 anti-Thioredoxin 2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-08
Question
We want to test anti-Thioredoxin 2 Monoclonal antibody M04586 on rat liver for research purposes, then I may be interested in using anti-Thioredoxin 2 Monoclonal antibody M04586 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The products we sell, including anti-Thioredoxin 2 Monoclonal antibody M04586, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-18
Question
I was wanting to use your anti-Thioredoxin 2 Monoclonal antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
S. Mitchell
Verified customer
Asked: 2016-12-08
Answer
It shows on the product datasheet, M04586 anti-Thioredoxin 2 Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-12-08
