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Product Info Summary
| SKU: | A04567-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-THC2 MASTL Antibody
SKU/Catalog Number
A04567-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-THC2 MASTL Antibody catalog # A04567-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-THC2 MASTL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04567-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MASTL. AA range:821-870
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04567-1 is reactive to MASTL in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
97319 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04567-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using THC2 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For MASTL (Source: Uniprot.org, NCBI)
Gene Name
MASTL
Full Name
Serine/threonine-protein kinase greatwall
Weight
97319 MW
Superfamily
protein kinase superfamily
Alternative Names
Serine/threonine-protein kinase greatwall;GW;GWL;hGWL;2.7.11.1;Microtubule-associated serine/threonine-protein kinase-like;MAST-L;MASTL;GW, GWL, THC2; Mastl|2700091H24Rik, C88295, GW, GWL, MAST-L, THC2|microtubule associated serine/threonine kinase-like|serine/threonine-protein kinase greatwall|microtubule-associated serine/threonine-protein kinase-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MASTL, check out the MASTL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MASTL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-THC2 MASTL Antibody (A04567-1)
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No publications found for A04567-1
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-THC2 MASTL Antibody
Question
Is a blocking peptide available for product anti-THC2 antibody (A04567-1)?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We do provide the blocking peptide for product anti-THC2 antibody (A04567-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-09
Question
I see that the anti-THC2 antibody A04567-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-31
Question
We are interested in to test anti-THC2 antibody A04567-1 on human amniotic fluid for research purposes, then I may be interested in using anti-THC2 antibody A04567-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Miller
Verified customer
Asked: 2020-01-30
Answer
The products we sell, including anti-THC2 antibody A04567-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-30
Question
See attached the WB image, lot number and protocol we used for amniotic fluid using anti-THC2 antibody A04567-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-14
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amniotic fluid using anti-THC2 antibody A04567-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-06
Question
Would anti-THC2 antibody A04567-1 work on goat WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Our lab technicians have not tested anti-THC2 antibody A04567-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-THC2 antibody A04567-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-06
Question
I was wanting to use your anti-THC2 antibody for WB for human amniotic fluid on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human amniotic fluid identification?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
You can see on the product datasheet, A04567-1 anti-THC2 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human amniotic fluid in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-02
Question
Would A04567-1 anti-THC2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
As indicated on the product datasheet, A04567-1 anti-THC2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-25
Question
Can you help my question with product A04567-1, anti-THC2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Patel
Verified customer
Asked: 2015-02-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04567-1 anti-THC2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-02-19
Question
We are currently using anti-THC2 antibody A04567-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on monkey tissues as well?
J. Mitchell
Verified customer
Asked: 2014-11-03
Answer
The anti-THC2 antibody (A04567-1) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-11-03
Question
Is this A04567-1 anti-THC2 antibody reactive to the isotypes of MASTL?
V. Thomas
Verified customer
Asked: 2014-10-22
Answer
The immunogen of A04567-1 anti-THC2 antibody is Synthesized peptide derived from the C-terminal region of human THC2. at AA rangle: 790-870. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-10-22
Question
Do you have a BSA free version of anti-THC2 antibody A04567-1 available?
A. Collins
Verified customer
Asked: 2014-03-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-THC2 antibody A04567-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-03-19
Question
Will anti-THC2 antibody A04567-1 work for WB with amniotic fluid?
C. Carter
Verified customer
Asked: 2013-11-08
Answer
According to the expression profile of amniotic fluid, MASTL is highly expressed in amniotic fluid. So, it is likely that anti-THC2 antibody A04567-1 will work for WB with amniotic fluid.
Boster Scientific Support
Answered: 2013-11-08
