Product Info Summary
SKU: | A08395 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ATF6B/Atf6 Beta Antibody
SKU/Catalog Number
A08395
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ATF6B/Atf6 Beta Antibody (Catalog # A08395). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF6B/Atf6 Beta Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08395)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ATF6B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08395 is reactive to ATF6B in Human
Reconstitution
Calculated molecular weight
76709 MW
Background of ATF6 beta
Transcriptional factor that acts in the unfolded protein response (UPR) pathway by activating UPR target genes induced during ER stress. Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAATN9CCAC[GA]-3') when NF-Y is bound to ERSE.
Min J., Genomics 30:149-156(1995).
Khanna A., Biochem. J. 319:81-89(1996).
Xie T., Genome Res. 13:2621-2636(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08395 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from COLO205 cells, using ATF6B antibody A08395.
Protein Target Info & Infographic
Gene/Protein Information For ATF6B (Source: Uniprot.org, NCBI)
Gene Name
ATF6B
Full Name
Cyclic AMP-dependent transcription factor ATF-6 beta
Weight
76709 MW
Superfamily
bZIP family
Alternative Names
Cyclic AMP-dependent transcription factor ATF-6 beta;cAMP-dependent transcription factor ATF-6 beta;Activating transcription factor 6 beta;ATF6-beta;Protein G13;cAMP response element-binding protein-related protein;Creb-rp;cAMP-responsive element-binding protein-like 1;Processed cyclic AMP-dependent transcription factor ATF-6 beta;ATF6B;CREBL1, G13; ATF6B CREB-RP, CREBL1, G13 activating transcription factor 6 beta cyclic AMP-dependent transcription factor ATF-6 beta|Creb-related protein|cAMP response element-binding protein-related protein|cAMP-dependent transcription factor ATF-6 beta|cAMP-responsive element-binding protein-like 1|protein G13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF6B, check out the ATF6B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF6B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATF6B/Atf6 Beta Antibody (A08395)
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No publications found for A08395
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Customer Q&As
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6 Customer Q&As for Anti-ATF6B/Atf6 Beta Antibody
Question
I see that the anti-ATF6B/Atf6 Beta antibody A08395 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-21
Question
Please see the WB image, lot number and protocol we used for tonsil using anti-ATF6B/Atf6 Beta antibody A08395. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-01
Question
Will anti-ATF6B/Atf6 Beta antibody A08395 work on zebrafish WB with tonsil?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
Our lab technicians have not tested anti-ATF6B/Atf6 Beta antibody A08395 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ATF6B/Atf6 Beta antibody A08395 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish tonsil in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-10
Question
I am interested in to test anti-ATF6B/Atf6 Beta antibody A08395 on human tonsil for research purposes, then I may be interested in using anti-ATF6B/Atf6 Beta antibody A08395 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
The products we sell, including anti-ATF6B/Atf6 Beta antibody A08395, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-25
Question
I was wanting to use your anti-ATF6B/Atf6 Beta antibody for WB for human tonsil on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human tonsil identification?
R. Kulkarni
Verified customer
Asked: 2018-02-15
Answer
As indicated on the product datasheet, A08395 anti-ATF6B/Atf6 Beta antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tonsil in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-15
Question
We are currently using anti-ATF6B/Atf6 Beta antibody A08395 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
The anti-ATF6B/Atf6 Beta antibody (A08395) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-28