Anti-TGM5 Antibody

Product Name

Anti-TGM5 Antibody

View all Transglutaminase 5 Antibodies

SKU/Catalog Number

A08563

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TGM5 Antibody (Catalog # A08563). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TGM5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TGM5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08563)

Host

Rabbit

Contents

TGM5 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TGM5 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TGM5. The immunogen is located within the last 50 amino acids of TGM5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

TGM5 antibody is predicted to not cross-react with other TGase protein family members. Multiple isoforms of TGM5 are known to exist.

Reactive Species

A08563 is reactive to TGM5 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

80778 MW

Background of Transglutaminase 5

Transglutaminases (TGM) are a family of structurally and functionally related Ca2+-dependent enzymes (TGases) that stabilize protein assemblies through the formation of gamma-glutamyl-epsilon lysine crosslinks. TGases influence numerous biological processes, including blood coagulation, cell differentiation, fertilization and apoptosis. TGM5 belongs to the transglutaminase superfamily and catalyzes the cross-linking of proteins between glutamine and lysine residues, often resulting in stabilization of protein assemblies. TGM5 is also expressed in the human hair follicle. Defects in TGM5 are associated with acral peeling skin syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08563 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TGM5 antibody can be used for detection of TGM5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For TGM5 (Source: Uniprot.org, NCBI)

Alternative Names

TGX, TGM6, TGMX, TGASE5, TGASEX, Protein-glutamine gamma-glutamyltransferase 5, Transglutaminase X, TGX TGM5 PSS2, TGASE5, TGASEX, TGM6, TGMX, TGX transglutaminase 5 protein-glutamine gamma-glutamyltransferase 5|TG(X)|TGase X|TGase-5|transglutaminase V|transglutaminase X

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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