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Key=sku, value=PB9448
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Key=created_at, value=2015-05-14 14:11:10
Key=updated_at, value=2024-09-26 00:46:31
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Key=name, value=Anti-Transglutaminase 2/TGM2 Antibody Picoband®
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Key=price, value=370.0000
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Key=meta_title, value=Transglutaminase 2/TGM2 Antibody
Key=meta_description, value=Boster Bio Anti-Transglutaminase 2/TGM2 Antibody Picoband® catalog # PB9448. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Figure 2. IHC analysis of TGM2 using anti-TGM2 antibody (PB9448).
TGM2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins.
Key=small_image_label, value=Figure 2. IHC analysis of TGM2 using anti-TGM2 antibody (PB9448).
TGM2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins.
Key=thumbnail_label, value=Figure 2. IHC analysis of TGM2 using anti-TGM2 antibody (PB9448).
TGM2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins.
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Key=size, value=100 μg/vial
Key=protein_name, value=Protein-glutamine gamma-glutamyltransferase 2
Key=gene_full_name, value=Protein-glutamine gamma-glutamyltransferase 2
Key=uniprot_id, value=P21980
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=sensitivity
Key=applications, value=IHC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
Key=predicted_reactivity
Key=source_company, value=Boster PB9448
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=77329 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Amino Acid Metabolism, Amino Acids, Apoptosis, Associated Proteins, Autoimmune, Calcium Binding Proteins, Calcium Signaling, Cancer, Cell Biology, Immune System Diseases, Immunology, Intracellular, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Signaling Pathway
Key=clonality, value=Polyclonal
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Key=rating_value, value=95.1
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Key=cj_related_products, value=Immune System Diseases:EKC1402^Synapse Organization:PA2153|M02229-1|EKC1575|PROTP21980
Key=description, value=Boster Bio Anti-Transglutaminase 2/TGM2 Antibody Picoband® catalog # PB9448. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Transglutaminase 2/TGM2 Antibody Picoband® catalog # PB9448. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Transglutaminase 2/TGM2 Antibody Picoband®
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=Tissue transglutaminase, also called TGC, is a 78-kDa, calcium dependent enzyme of the protein-glutamine gamma-glutamyltransferases family. By fluorescence in situ hybridization (FISH), this gene is mapped in 20q11.23. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease.
Key=gene_name, value=TGM2
Key=synonyms, value=Protein-glutamine gamma-glutamyltransferase 2;2.3.2.13;Tissue transglutaminase;Transglutaminase C;TG (C);TGC;TGase C;Transglutaminase H;TGase H;Transglutaminase-2;TGase-2;TGM2;
Key=application_details, value=Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat

Key=sequence_similarities, value=Belongs to the transglutaminase superfamily. Transglutaminase family.
Key=protein_function, value= Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Key=application_notes, value=WB: The detection limit for TGM2 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 19509|sc 19510|sc 20621|sc 34543|sc 48314|sc 48315|sc 48370|sc 48387|sc 73612
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the N-terminus of human TGM2, different from the related mouse sequence by five amino acids.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=77 kDa
Key=custom_attribute_2, value=WB: Rat Liver Tissue, Rat Ovary Tissue, HELA Whole Cell,
IHC: mouse spleen tissue, rat spleen tissue, human intestinal cancer tissue, human mammary cancer tissue
IHC-F: mouse spleen tissue, rat spleen tissue, human placenta tissue
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Anti-Transglutaminase 2/TGM2 Antibody Picoband®

Transglutaminase 2/TGM2 antibody

Boster Bio Anti-Transglutaminase 2/TGM2 Antibody Picoband® catalog # PB9448. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9448
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Transglutaminase 2/TGM2 Antibody Picoband®

View all Transglutaminase 2/TGM2 Antibodies

SKU/Catalog Number

PB9448

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Transglutaminase 2/TGM2 Antibody Picoband® catalog # PB9448. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Transglutaminase 2/TGM2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9448)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human TGM2, different from the related mouse sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9448 is reactive to TGM2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

77 kDa

Calculated molecular weight

77329 MW

Background of Transglutaminase 2/TGM2

Tissue transglutaminase, also called TGC, is a 78-kDa, calcium dependent enzyme of the protein-glutamine gamma-glutamyltransferases family. By fluorescence in situ hybridization (FISH), this gene is mapped in 20q11.23. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9448 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: Rat Liver Tissue, Rat Ovary Tissue, HELA Whole Cell,
IHC: mouse spleen tissue, rat spleen tissue, human intestinal cancer tissue, human mammary cancer tissue
IHC-F: mouse spleen tissue, rat spleen tissue, human placenta tissue

Validation Images & Assay Conditions

Gene/Protein Information For TGM2 (Source: Uniprot.org, NCBI)

Gene Name

TGM2

Full Name

Protein-glutamine gamma-glutamyltransferase 2

Weight

77329 MW

Superfamily

transglutaminase superfamily

Alternative Names

Protein-glutamine gamma-glutamyltransferase 2;2.3.2.13;Tissue transglutaminase;Transglutaminase C;TG (C);TGC;TGase C;Transglutaminase H;TGase H;Transglutaminase-2;TGase-2;TGM2; TGM2 TG(C), TGC transglutaminase 2 protein-glutamine gamma-glutamyltransferase 2|C polypeptide, protein-glutamine-gamma-glutamyltransferase|TGase C|TGase H|TGase-2|tissue transglutaminase|transglutaminase C|transglutaminase H

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TGM2, check out the TGM2 Infographic

TGM2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TGM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Transglutaminase 2/TGM2 Antibody Picoband®

Question

We are currently using anti-Transglutaminase 2/TGM2 antibody PB9448 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Z. Williams

Verified customer

Asked: 2013-01-23

Answer

The anti-Transglutaminase 2/TGM2 antibody (PB9448) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-01-23

Order DetailsPrice
PB9448

100μg

$370
PB9448-10ug

10μg sample (liquid)

$99
PB9448-Biotin

100 μg Biotin conjugated

$570
PB9448-Cy3

100 μg Cy3 conjugated

$570
PB9448-Dylight488

100 μg Dylight488 conjugated

$570
PB9448-Dylight550

100 μg Dylight550 conjugated

$570
PB9448-Dylight594

100 μg Dylight594 conjugated

$570
PB9448-FITC

100 μg FITC conjugated

$570
PB9448-HRP

100 μg HRP conjugated

$570
PB9448-APC

100 μg APC conjugated

$670
PB9448-PE

100 μg PE conjugated

$670
PB9448-iFluor647

100 μg iFluor647 conjugated

$670
PB9448-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9448
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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