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Product Info Summary
| SKU: | M02374 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-TERF2IP Antibody
SKU/Catalog Number
M02374
Size
50 µl
Description
Boster Bio Anti-TERF2IP Antibody (Catalog # M02374). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TERF2IP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02374)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1664CT564.52.88
Isotype
IgG1,k
Immunogen
This TERF2IP antibody is generated from a mouse immunized with a recombinant protein from human TERF2IP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02374 is reactive to TERF2IP in Human
Reconstitution
Calculated molecular weight
44260 Da
Background of TERF2IP
Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'- TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02374 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-TERF2IP Antibody at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M02374 staining TE2IP in human testis tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing Hela cells stained with M02374 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M02374, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For TERF2IP (Source: Uniprot.org, NCBI)
Gene Name
TERF2IP
Full Name
Telomeric repeat-binding factor 2-interacting protein 1
Weight
44260 Da
Superfamily
RAP1 family
Alternative Names
Telomeric repeat-binding factor 2-interacting protein 1, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric protein 1, Dopamine receptor-interacting protein 5, Repressor/activator protein 1 homolog, RAP1 homolog, hRap1, TERF2IP, DRIP5, RAP1 TERF2IP DRIP5, RAP1 TERF2 interacting protein telomeric repeat-binding factor 2-interacting protein 1|TERF2-interacting telomeric protein 1|TRF2-interacting telomeric RAP1 protein|dopamine receptor-interacting protein 5|repressor/activator protein 1 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TERF2IP, check out the TERF2IP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TERF2IP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TERF2IP Antibody (M02374)
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