Anti-TEM1 CD248 Antibody

Product Name

Anti-TEM1 CD248 Antibody

View all Endosialin/CD248/TEM1 Antibodies

SKU/Catalog Number

A04735

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TEM1 CD248 Antibody (Catalog # A04735). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

TEM1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TEM1 CD248 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04735)

Host

Rabbit

Contents

TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-TEM-1 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TEM-1. The immunogen is located within the last 50 amino acids of TEM-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.

Reactive Species

A04735 is reactive to CD248 in Human

Applications

A04735 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

80859 MW

Background of Endosialin/CD248/TEM1

Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.5-2 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western Blot Validation in Human Colon Tissue
Loading: 15 μg of lysates per lane.Antibodies: TEM-1 A04735, (A, 0.5 μg/mL; B, 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: TEM-1 A04735, (2 μg/mL), TEM-1, ( 1μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane. Antibodies: TEM-1 A04735, (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Immunohistochemistry Validation of TEM-1 in Human Colon Tissue
Immunohistochemical analysis of paraffin-embedded Human Colon tissue using anti-TEM-1 antibody (A04735) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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Immunofluorescence Validation of TEM-1 in Human Colon Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with A04735 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Gene/Protein Information For CD248 (Source: Uniprot.org, NCBI)

Alternative Names

2610111G01Rik; CD164 sialomucin-like 1; CD164L1; CD248 antigen; CD248 antigen, endosialin; CD248 molecule, endosialin; CD248; Endosialin; MGC119479; TEM1; TEM1MGC119478; Tumor endothelial marker 1 CD248 CD164L1, TEM1 CD248 molecule endosialin|2610111G01Rik|CD164 sialomucin-like 1|CD248 antigen, endosialin|CD248 molecule, endosialin|tumor endothelial marker 1

For more info on CD248, check out the CD248 Infographic

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In this infographic, you will see the following information for CD248: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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