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Product Info Summary
| SKU: | A12051 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Tectonic-3 TCTN3 Antibody
SKU/Catalog Number
A12051
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Tectonic-3 TCTN3 Antibody (Catalog # A12051). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TCTN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Tectonic-3 TCTN3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12051)
Host
Rabbit
Contents
TCTN3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TCTN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TCTN3. The immunogen is located within amino acids 540 - 590 of TCTN3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of SGSM1 are known to exist; SGSM1 antibody will detect all four isoforms.
Reactive Species
A12051 is reactive to TCTN3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
66157 MW
Background of TCTN3
TCTN3 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN3 is not known, it is 58% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12051 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TCTN3 antibody can be used for detection of TCTN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TCTN3 in HeLa cell lysate with TCTN3 antibody at (A) 1 and (B) 2μg/mL.
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Immunocytochemistry of TCTN3 in HeLa cells with TCTN3 antibody at 4 μg/mL.
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Immunofluorescence of TCTN3 in HeLa cells with TCTN3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TCTN3 (Source: Uniprot.org, NCBI)
Gene Name
TCTN3
Full Name
Tectonic-3
Weight
66157 MW
Superfamily
tectonic family
Alternative Names
OFD4, TECT3, JBTS18, C10orf61, PSEC0041, UNQ1881/PRO4324, Tectonic-3 TCTN3 C10orf61, JBTS18, OFD4, TECT3 tectonic family member 3 tectonic-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TCTN3, check out the TCTN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TCTN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tectonic-3 TCTN3 Antibody (A12051)
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