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Product Info Summary
| SKU: | M03414 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TCTP Rabbit Monoclonal Antibody
SKU/Catalog Number
M03414
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TCTP Rabbit Monoclonal Antibody catalog # M03414. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCTP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03414)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACDD-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TCTP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03414 is reactive to TPT1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated molecular weight
250537 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03414 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of TCTP Receptor expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For TPT1 (Source: Uniprot.org, NCBI)
Gene Name
TPT1
Full Name
Translationally-controlled tumor protein
Weight
250537 MW
Superfamily
TCTP family
Alternative Names
Chondroitin sulfate proteoglycan 4;Chondroitin sulfate proteoglycan NG2;Melanoma chondroitin sulfate proteoglycan;Melanoma-associated chondroitin sulfate proteoglycan;CSPG4;MCSP; TPT1 HRF, TCTP, p02, p23 tumor protein, translationally-controlled 1 translationally-controlled tumor protein|fortilin|histamine-releasing factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPT1, check out the TPT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TCTP Rabbit Monoclonal Antibody (M03414)
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No publications found for M03414
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Customer Q&As
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8 Customer Q&As for Anti-TCTP Rabbit Monoclonal Antibody
Question
Does anti-TCTP Monoclonal antibody M03414 work on primate WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Our lab technicians have not validated anti-TCTP Monoclonal antibody M03414 on primate. You can run a BLAST between primate and the immunogen sequence of anti-TCTP Monoclonal antibody M03414 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-16
Question
Is there a BSA free version of anti-TCTP Monoclonal antibody M03414 available?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TCTP Monoclonal antibody M03414 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-11
Question
We are currently using anti-TCTP Monoclonal antibody M03414 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
The anti-TCTP Monoclonal antibody (M03414) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-29
Question
What sample was used in your WB images for M03414 antibody?
Verified customer
Asked: 2019-01-10
Answer
The sample used for the Anti-TCTP Rabbit Monoclonal Antibody M03414 WB images is HeLa cell lysate.
Boster Scientific Support
Answered: 2019-01-13
Question
I was wanting to use your anti-TCTP Monoclonal antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
As indicated on the product datasheet, M03414 anti-TCTP Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-30
Question
Is a blocking peptide available for product anti-TCTP Monoclonal antibody (M03414)?
J. Zhao
Verified customer
Asked: 2018-06-07
Answer
We do provide the blocking peptide for product anti-TCTP Monoclonal antibody (M03414). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-07
Question
I see that the anti-TCTP Monoclonal antibody M03414 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-03
Question
Will M03414 anti-TCTP Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Jackson
Verified customer
Asked: 2016-09-19
Answer
You can see on the product datasheet, M03414 anti-TCTP Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-09-19
