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Product Info Summary
| SKU: | A01375 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SLCO1B1/Oatp2 Antibody
SKU/Catalog Number
A01375
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SLCO1B1/Oatp2 Antibody (Catalog # A01375). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SLCO1B1/Oatp2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01375)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SLCO1B1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01375 is reactive to SLCO1B1 in Human
Reconstitution
Calculated molecular weight
76449 MW
Background of SLCO1B1
Mediates the Na+-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. Involved in the clearance of bile acids and organic anions from the liver.
Kirchgessner T.G., J. Biol. Chem. 274:37161-37168(1999).
Keppler D., J. Biol. Chem. 275:23161-23168(2000).
Venter J.C., Submitted (JUL-2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01375 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO205 and Jurkat cells, using SLCO1B1 antibody A01375.
Protein Target Info & Infographic
Gene/Protein Information For SLCO1B1 (Source: Uniprot.org, NCBI)
Gene Name
SLCO1B1
Full Name
Solute carrier organic anion transporter family member 1B1
Weight
76449 MW
Superfamily
organo anion transporter (TC 2.A.60) family
Alternative Names
Solute carrier organic anion transporter family member 1B1;Liver-specific organic anion transporter 1;LST-1;OATP-C;Sodium-independent organic anion-transporting polypeptide 2;OATP-2;Solute carrier family 21 member 6;SLCO1B1;LST1, OATP1B1, OATP2, OATPC, SLC21A6; SLCO1B1 HBLRR, LST-1, LST1, OATP-C, OATP1B1, OATP2, OATPC, SLC21A6 solute carrier organic anion transporter family member 1B1 solute carrier organic anion transporter family member 1B1|OATP-2|liver-specific organic anion transporter 1|sodium-independent organic anion-transporting polypeptide 2|solute carrier family 21 (organic anion transporter), member 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLCO1B1, check out the SLCO1B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLCO1B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SLCO1B1/Oatp2 Antibody (A01375)
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No publications found for A01375
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Customer Q&As
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7 Customer Q&As for Anti-SLCO1B1/Oatp2 Antibody
Question
My question regarding product A01375, anti-SLCO1B1/Oatp2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01375 anti-SLCO1B1/Oatp2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-25
Question
I was wanting to use your anti-SLCO1B1/Oatp2 antibody for WB for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
As indicated on the product datasheet, A01375 anti-SLCO1B1/Oatp2 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-11
Question
Is there a BSA free version of anti-SLCO1B1/Oatp2 antibody A01375 available?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SLCO1B1/Oatp2 antibody A01375 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-30
Question
Does anti-SLCO1B1/Oatp2 antibody A01375 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
According to the expression profile of liver, SLCO1B1 is highly expressed in liver. So, it is likely that anti-SLCO1B1/Oatp2 antibody A01375 will work for WB with liver.
Boster Scientific Support
Answered: 2019-01-28
Question
Is this A01375 anti-SLCO1B1/Oatp2 antibody reactive to the isotypes of SLCO1B1?
N. Krishna
Verified customer
Asked: 2016-10-25
Answer
The immunogen of A01375 anti-SLCO1B1/Oatp2 antibody is Synthesized peptide derived from internal of human SLCO1B1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-10-25
Question
We are currently using anti-SLCO1B1/Oatp2 antibody A01375 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
R. Jackson
Verified customer
Asked: 2015-09-02
Answer
The anti-SLCO1B1/Oatp2 antibody (A01375) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-02
Question
Does A01375 anti-SLCO1B1/Oatp2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Anderson
Verified customer
Asked: 2013-10-28
Answer
It shows on the product datasheet, A01375 anti-SLCO1B1/Oatp2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-10-28
