Anti-TCR alpha/TRAC Antibody Picoband®

TRAC antibody

Boster Bio Anti-TCR alpha/TRAC Antibody Picoband® catalog # A05315. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A05315
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC, WB

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Product Name

Anti-TCR alpha/TRAC Antibody Picoband®

View all TRAC Antibodies

SKU/Catalog Number

A05315

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TCR alpha/TRAC Antibody Picoband® catalog # A05315. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TCR alpha/TRAC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05315)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human TCR alpha recombinant protein (Position: I1-L114).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05315 is reactive to TRAC in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

45 kDa

Calculated molecular weight

39411 MW

Background of TRAC

T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor alpha and delta loci. Both the alpha and delta loci include V (variable), J (joining), and C (constant) segments and the delta locus also includes diversity (D) segments. The delta locus is situated within the alpha locus, between the alpha V and J segments. During T cell development, the delta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The alpha chain is synthesized by recombination joining a single V segment with a J segment. For both chains, the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Five variable segments can be used in either alpha or delta chains and are described by TRAV/DV symbols. Several V and J segments of the alpha locus are known to be incapable of encoding a protein and are considered pseudogenes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A05315 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/m
Flow Cytometry(Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml

Positive Control

WB: human Jurkat whole cell
IHC: human tonsil tissue, human urothelium carcinoma tissue, mouse thymus tissue, mouse thymus tissue, rat thymus tissue
FCM: Jurkat cell

Validation Images & Assay Conditions

Gene/Protein Information For TRAC (Source: Uniprot.org, NCBI)

Gene Name

TRAC

Full Name

T cell receptor alpha constant

Weight

39411 MW

Alternative Names

T-cell receptor alpha chain C region; TRAC; TCRA TRAC IMD7, TCRA, TRA, TRCA T cell receptor alpha constant

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRAC, check out the TRAC Infographic

TRAC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRAC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-TCR alpha/TRAC Antibody Picoband®

Question

Does anti-TCR alpha/TRAC antibody A05315 work on dog Flow Cytometry with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

Our lab technicians have not tested anti-TCR alpha/TRAC antibody A05315 on dog. You can run a BLAST between dog and the immunogen sequence of anti-TCR alpha/TRAC antibody A05315 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog leukemic t-cell in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-29

Question

I see that the anti-TCR alpha/TRAC antibody A05315 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-12-11

Question

Do you have a BSA free version of anti-TCR alpha/TRAC antibody A05315 available?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TCR alpha/TRAC antibody A05315 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-07

Question

Does A05315 anti-TCR alpha/TRAC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-03-28

Answer

As indicated on the product datasheet, A05315 anti-TCR alpha/TRAC antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-03-28

Question

We are currently using anti-TCR alpha/TRAC antibody A05315 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

R. Carter

Verified customer

Asked: 2018-01-04

Answer

The anti-TCR alpha/TRAC antibody (A05315) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-04

Question

Is a blocking peptide available for product anti-TCR alpha/TRAC antibody (A05315)?

W. Wu

Verified customer

Asked: 2016-08-11

Answer

We do provide the blocking peptide for product anti-TCR alpha/TRAC antibody (A05315). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-08-11

Question

I was wanting to use your anti-TCR alpha/TRAC antibody for ELISA for mouse leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukemic t-cell identification?

N. Parker

Verified customer

Asked: 2015-07-07

Answer

You can see on the product datasheet, A05315 anti-TCR alpha/TRAC antibody has been tested for ELISA, Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-07-07

Order DetailsPrice
A05315

100μg

$370
A05315-10ug

10μg sample (liquid)

$99
A05315-Biotin

100 μg Biotin conjugated

$570
A05315-Cy3

100 μg Cy3 conjugated

$570
A05315-Dylight488

100 μg Dylight488 conjugated

$570
A05315-Dylight550

100 μg Dylight550 conjugated

$570
A05315-Dylight594

100 μg Dylight594 conjugated

$570
A05315-FITC

100 μg FITC conjugated

$570
A05315-HRP

100 μg HRP conjugated

$570
A05315-APC

100 μg APC conjugated

$670
A05315-PE

100 μg PE conjugated

$670
A05315-iFluor647

100 μg iFluor647 conjugated

$670
A05315-carrier-free

Carrier Free

$370

More conjugates/formats

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A05315
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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