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Product Info Summary
| SKU: | A02653 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-TCOF1/Treacle Antibody
View all Treacher Collins syndrome protein Antibodies
SKU/Catalog Number
A02653
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TCOF1/Treacle Antibody (Catalog# A02653). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCOF1/Treacle Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02653)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TCOF1. Immunogen sequence location: 41-90
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02653 is reactive to TCOF1 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
152106 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02653 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat, 293, HeLa cells, using TCOF1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TCOF1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For TCOF1 (Source: Uniprot.org, NCBI)
Gene Name
TCOF1
Full Name
Treacle protein
Weight
152106 MW
Alternative Names
TCOF1;Treacle protein;Treacher Collins syndrome protein TCOF1 MFD1, TCS, TCS1, treacle treacle ribosome biogenesis factor 1 treacle protein|Treacher Collins syndrome protein|Treacher Collins-Franceschetti syndrome 1|nucleolar trafficking phosphoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TCOF1, check out the TCOF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TCOF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TCOF1/Treacle Antibody (A02653)
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No publications found for A02653
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-TCOF1/Treacle Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-TCOF1/Treacle antibody A02653. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-27
Question
Is there a BSA free version of anti-TCOF1/Treacle antibody A02653 available?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TCOF1/Treacle antibody A02653 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-27
Question
My question regarding product A02653, anti-TCOF1/Treacle antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Bhatt
Verified customer
Asked: 2017-06-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02653 anti-TCOF1/Treacle antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-05
Question
We are currently using anti-TCOF1/Treacle antibody A02653 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
B. Mangal
Verified customer
Asked: 2015-07-14
Answer
The anti-TCOF1/Treacle antibody (A02653) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-07-14
Question
Would anti-TCOF1/Treacle antibody A02653 work for IHC with cervix carcinoma erythroleukemia?
C. Evans
Verified customer
Asked: 2014-12-12
Answer
According to the expression profile of cervix carcinoma erythroleukemia, TCOF1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-TCOF1/Treacle antibody A02653 will work for IHC with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2014-12-12
