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Product Info Summary
| SKU: | A00302 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TATA binding protein TBP Antibody Picoband®
View all TATA binding protein TBP Antibodies
SKU/Catalog Number
A00302
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TATA binding protein TBP Antibody Picoband® catalog # A00302. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TATA binding protein TBP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00302)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Tbp recombinant protein (Position: L38-A107).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00302 is reactive to Tbp in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
50495 MW
Background of TATA binding protein TBP
TBP, TATA-binding protein, is a general transcription factor that binds specifically to a DNA sequence called the TATA box. The TBP gene is mapped to 6q27. This DNA sequence is found about 35 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00302 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat testis tissue, rat ovary tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Tbp using anti-Tbp antibody (A00302).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysate,
Lane 2: rat ovary tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tbp antigen affinity purified polyclonal antibody (Catalog # A00302) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tbp at approximately 38KD. The expected band size for Tbp is at 38KD.
Protein Target Info & Infographic
Gene/Protein Information For Tbp (Source: Uniprot.org, NCBI)
Gene Name
Tbp
Full Name
TATA-box-binding protein
Weight
50495 MW
Superfamily
TBP family
Alternative Names
TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit; Tbp; Tfiid TBP GTF2D, GTF2D1, HDL4, SCA17, TFIID TATA-box binding protein TATA-box-binding protein|TATA sequence-binding protein|TATA-box binding protein N-terminal domain|TATA-box factor|transcription initiation factor TFIID TBP subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tbp, check out the Tbp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tbp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TATA binding protein TBP Antibody Picoband® (A00302)
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Customer Q&As
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16 Customer Q&As for Anti-TATA binding protein TBP Antibody Picoband®
Question
We were satisfied with the WB result of your anti-TATA binding protein TBP antibody. However we have been able to see positive staining in forebrain nucleus. using this antibody. Is that expected? Could you tell me where is TBP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
From literature, forebrain does express TBP. Generally TBP expresses in nucleus. Regarding which tissues have TBP expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2194289
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-28
Question
I was wanting to use your anti-TATA binding protein TBP antibody for ELISA for mouse forebrain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse forebrain identification?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
As indicated on the product datasheet, A00302 anti-TATA binding protein TBP antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse forebrain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-16
Question
See below the WB image, lot number and protocol we used for forebrain using anti-TATA binding protein TBP antibody A00302. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-20
Question
I see that the anti-TATA binding protein TBP antibody A00302 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-19
Question
We have seen staining in mouse uterus. Are there any suggestions? Is anti-TATA binding protein TBP antibody supposed to stain uterus positively?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
Based on literature uterus does express TBP. Based on Uniprot.org, TBP is expressed in forebrain, fibroblast, uterus, among other tissues. Regarding which tissues have TBP expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2194289
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-04
Question
We have tried in the past anti-TATA binding protein TBP antibody for ELISA on fibroblast in a previous experiment. I am using rat, and We want to use the antibody for WB next. We need examining fibroblast as well as uterus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
I viewed the website and datasheets of our anti-TATA binding protein TBP antibody and it seems that A00302 has been tested on rat in both ELISA and WB. Thus A00302 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-06
Question
Is there a BSA free version of anti-TATA binding protein TBP antibody A00302 available?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TATA binding protein TBP antibody A00302 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-31
Question
Would anti-TATA binding protein TBP antibody A00302 work for ELISA with forebrain?
Verified Customer
Verified customer
Asked: 2019-02-26
Answer
According to the expression profile of forebrain, TBP is highly expressed in forebrain. So, it is likely that anti-TATA binding protein TBP antibody A00302 will work for ELISA with forebrain.
Boster Scientific Support
Answered: 2019-02-26
Question
I am interested in to test anti-TATA binding protein TBP antibody A00302 on mouse forebrain for research purposes, then I may be interested in using anti-TATA binding protein TBP antibody A00302 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
The products we sell, including anti-TATA binding protein TBP antibody A00302, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-03
Question
Is a blocking peptide available for product anti-TATA binding protein TBP antibody (A00302)?
A. Krishna
Verified customer
Asked: 2017-05-04
Answer
We do provide the blocking peptide for product anti-TATA binding protein TBP antibody (A00302). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-04
Question
I am interested in using your anti-TATA binding protein TBP antibody for r-mmu-9018519; estrogen-dependent gene expression studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
D. Collins
Verified customer
Asked: 2017-02-08
Answer
Thank you for your inquiry. This A00302 anti-TATA binding protein TBP antibody is validated on rat ovary tissue, testis tissue, tissue lysate. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-02-08
Question
We are currently using anti-TATA binding protein TBP antibody A00302 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
C. Krishna
Verified customer
Asked: 2015-10-16
Answer
The anti-TATA binding protein TBP antibody (A00302) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-10-16
Question
Would A00302 anti-TATA binding protein TBP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Williams
Verified customer
Asked: 2015-05-06
Answer
You can see on the product datasheet, A00302 anti-TATA binding protein TBP antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-05-06
Question
Is this A00302 anti-TATA binding protein TBP antibody reactive to the isotypes of TBP?
D. Evans
Verified customer
Asked: 2014-08-18
Answer
The immunogen of A00302 anti-TATA binding protein TBP antibody is E. coli-derived mouse Tbp recombinant protein (Position: L38-A107). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-08-18
Question
My question regarding product A00302, anti-TATA binding protein TBP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Thomas
Verified customer
Asked: 2013-12-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00302 anti-TATA binding protein TBP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-12-16
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for forebrain using anti-TATA binding protein TBP antibody A00302. Let me know if you need anything else.
F. Krishna
Verified customer
Asked: 2013-11-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-11-18
