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Product Info Summary
| SKU: | A04524 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Bovine, Chicken, Human, Mouse, Pig, Rat, Zebrafish |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-TBL1XR1/Tblr1 Monoclonal Antibody
SKU/Catalog Number
A04524
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TBL1XR1/Tblr1 Monoclonal Antibody catalog # A04524. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Human, Mouse, Pig, Rat, Zebrafish.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TBL1XR1/Tblr1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04524)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
3F8
Isotype
IgG
Immunogen
Purified recombinant human TBL1XR1 protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04524 is reactive to TBL1XR1 in Bovine, Chicken, Human, Mouse, Pig, Rat, Zebrafish
Applications
A04524 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
55595 MW
Background of TBLR1
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Pennica D., Swanson T.A., Welsh J.W. J.Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
IHC, 1:500-1:1000
IF, 1:100-1:500
Validation Images & Assay Conditions
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Immunohistochemistry (IHC) analysis of paraffin-embedded huma breast cancer using TBL1XR1 Monoclonal antibody.
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Western Blot (WB) analysis using TBL1XR1 Monoclonal antibody against Mouse Brain, Mouse Heart, K562 cell lysate.
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Immunofluorescence (IF) analysis of HeLa cells using TBL1XR1 Monoclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For TBL1XR1 (Source: Uniprot.org, NCBI)
Gene Name
TBL1XR1
Full Name
F-box-like/WD repeat-containing protein TBL1XR1
Weight
55595 MW
Superfamily
WD repeat EBI family
Alternative Names
C21; DC42; F-box-like/WD repeat-containing protein TBL1XR1; FLJ12894; IRA1Transducin beta-like 1X-related protein 1; Nuclear receptor corepressor/HDAC3 complex subunit TBLR1; nuclear receptor co-repressor/HDAC3 complex subunit; TBLR1TBL1-related protein 1; transducin (beta)-like 1 X-linked receptor 1; transducin (beta)-like 1X-linked receptor 1 TBL1XR1 C21, DC42, IRA1, MRD41, TBLR1 TBL1X receptor 1 F-box-like/WD repeat-containing protein TBL1XR1|TBL1-related protein 1|nuclear receptor co-repressor/HDAC3 complex subunit|nuclear receptor corepressor/HDAC3 complex subunit TBLR1|transducin beta like 1 X-linked receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TBL1XR1, check out the TBL1XR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TBL1XR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TBL1XR1/Tblr1 Monoclonal Antibody (A04524)
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No publications found for A04524
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-TBL1XR1/Tblr1 Monoclonal Antibody
Question
See below the WB image, lot number and protocol we used for embryonic kidney using anti-TBL1XR1/Tblr1 Monoclonal antibody A04524. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-24
Question
Is this A04524 anti-TBL1XR1/Tblr1 Monoclonal antibody reactive to the isotypes of TBL1XR1?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The immunogen of A04524 anti-TBL1XR1/Tblr1 Monoclonal antibody is Purified recombinant human TBL1XR1 protein fragments expressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-28
Question
We are currently using anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 for pig tissue, and we are content with the WB results. The species of reactivity given in the datasheet says bovine, chicken, human, mouse, pig, rat, zebrafish. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The anti-TBL1XR1/Tblr1 Monoclonal antibody (A04524) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
Question
I see that the anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-16
Question
I was wanting to use your anti-TBL1XR1/Tblr1 Monoclonal antibody for WB for mouse embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryonic kidney identification?
T. Carter
Verified customer
Asked: 2019-02-14
Answer
As indicated on the product datasheet, A04524 anti-TBL1XR1/Tblr1 Monoclonal antibody has been validated for IF, IHC, WB on bovine, chicken, human, mouse, pig, rat, zebrafish tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-14
Question
Does anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 work on pig IF with forebrain?
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
Our lab technicians have not tested anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 on pig. You can run a BLAST between pig and the immunogen sequence of anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig forebrain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-TBL1XR1/Tblr1 Monoclonal antibody A04524. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-06
Question
Would anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 work for WB with embryonic kidney?
Verified Customer
Verified customer
Asked: 2018-11-13
Answer
According to the expression profile of embryonic kidney, TBL1XR1 is highly expressed in embryonic kidney. So, it is likely that anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2018-11-13
Question
We need to test anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 on mouse embryonic kidney for research purposes, then I may be interested in using anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Krishna
Verified customer
Asked: 2017-05-16
Answer
The products we sell, including anti-TBL1XR1/Tblr1 Monoclonal antibody A04524, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-16
Question
Do you have a BSA free version of anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 available?
T. Walker
Verified customer
Asked: 2016-07-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TBL1XR1/Tblr1 Monoclonal antibody A04524 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-07-27
Question
Is a blocking peptide available for product anti-TBL1XR1/Tblr1 Monoclonal antibody (A04524)?
P. Lewis
Verified customer
Asked: 2013-12-12
Answer
We do provide the blocking peptide for product anti-TBL1XR1/Tblr1 Monoclonal antibody (A04524). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-12-12
Question
Does A04524 anti-TBL1XR1/Tblr1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Miller
Verified customer
Asked: 2013-07-08
Answer
As indicated on the product datasheet, A04524 anti-TBL1XR1/Tblr1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-07-08
Question
Can you help my question with product A04524, anti-TBL1XR1/Tblr1 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Collins
Verified customer
Asked: 2013-02-14
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04524 anti-TBL1XR1/Tblr1 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-14
