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Product Info Summary
| SKU: | A10516 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TBC1 domain family member 3 TBC1D3 Antibody
SKU/Catalog Number
A10516
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TBC1 domain family member 3 TBC1D3 Antibody (Catalog # A10516). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TBC1 domain family member 3 TBC1D3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10516)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human TBC1D3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10516 is reactive to TBC1D3 in Human
Reconstitution
Calculated molecular weight
62187 MW
Background of TBC1D3
Acts as a GTPase activating protein for RAB5. Does not act on RAB4 or RAB11.
Pei L., Cancer Res. 62:5420-5424(2002).
Wiemann S., Genome Res. 11:422-435(2001).
Onno M., DNA Cell Biol. 12:107-118(1993).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10516 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa cells, using TBC1D3 antibody A10516.
Protein Target Info & Infographic
Gene/Protein Information For TBC1D3 (Source: Uniprot.org, NCBI)
Gene Name
TBC1D3
Full Name
TBC1 domain family member 3
Weight
62187 MW
Alternative Names
TBC1 domain family member 3;Prostate cancer gene 17 protein;Protein TRE17-alpha;Rab GTPase-activating protein PRC17;TBC1D3;PRC17, TBC1D3A; TBC1D3 PRC17A, TBC1D3C, TBC1D3D, TBC1D3F, TBC1D3L, TBC1D3 TBC1 domain family member 3 TBC1 domain family member 3|Rab GTPase-activating protein PRC17|TBC1 domain family member 3C|TBC1 domain family member 3L|TBC1 domain family, member 3F|prostate cancer gene 17 protein|protein TRE17-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TBC1D3, check out the TBC1D3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TBC1D3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TBC1 domain family member 3 TBC1D3 Antibody (A10516)
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No publications found for A10516
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Customer Q&As
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7 Customer Q&As for Anti-TBC1 domain family member 3 TBC1D3 Antibody
Question
Is there a BSA free version of anti-TBC1D3 antibody A10516 available?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TBC1D3 antibody A10516 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-11
Question
My question regards to test anti-TBC1D3 antibody A10516 on human adipose tissue for research purposes, then I may be interested in using anti-TBC1D3 antibody A10516 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The products we sell, including anti-TBC1D3 antibody A10516, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-24
Question
Would anti-TBC1D3 antibody A10516 work for WB with adipose tissue?
Z. Singh
Verified customer
Asked: 2019-07-01
Answer
According to the expression profile of adipose tissue, TBC1D3 is highly expressed in adipose tissue. So, it is likely that anti-TBC1D3 antibody A10516 will work for WB with adipose tissue.
Boster Scientific Support
Answered: 2019-07-01
Question
I see that the anti-TBC1D3 antibody A10516 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-08
Question
I have a question about product A10516, anti-TBC1D3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Wu
Verified customer
Asked: 2018-03-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10516 anti-TBC1D3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-28
Question
We are currently using anti-TBC1D3 antibody A10516 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
M. Jackson
Verified customer
Asked: 2015-12-07
Answer
The anti-TBC1D3 antibody (A10516) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-12-07
Question
Is this A10516 anti-TBC1D3 antibody reactive to the isotypes of TBC1D3?
N. Carter
Verified customer
Asked: 2014-10-06
Answer
The immunogen of A10516 anti-TBC1D3 antibody is Synthesized peptide derived from internal of human TBC1D3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-10-06
