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Product Info Summary
| SKU: | A18662 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Ret finger protein-like 4A RFPL4A Antibody
SKU/Catalog Number
A18662
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Ret finger protein-like 4A RFPL4A Antibody (Catalog # A18662). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ret finger protein-like 4A RFPL4A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18662)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RFPL4A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A18662 is reactive to RFPL4A in Human
Reconstitution
Calculated molecular weight
32238 MW
Background of Rfpl4a
May be involved in transcriptional regulation.
Ota T., Nat. Genet. 36:40-45(2004).
Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A18662 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HUVEC cells, using RFPL4A antibody A18662.
Protein Target Info & Infographic
Gene/Protein Information For RFPL4A (Source: Uniprot.org, NCBI)
Gene Name
RFPL4A
Full Name
Ret finger protein-like 4A
Weight
32238 MW
Alternative Names
Ret finger protein-like 4A;RING finger protein 210;RFPL4A;RFPL4, RNF210; Rfpl4a|Rfpl4|ret finger protein-like 4A|ret finger protein-like 4A|ret finger protein-like 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RFPL4A, check out the RFPL4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RFPL4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ret finger protein-like 4A RFPL4A Antibody (A18662)
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No publications found for A18662
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Ret finger protein-like 4A RFPL4A Antibody
Question
Does A18662 anti-RFPL4A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
You can see on the product datasheet, A18662 anti-RFPL4A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-02
Question
My question regarding product A18662, anti-RFPL4A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A18662 anti-RFPL4A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-19
Question
Is there a BSA free version of anti-RFPL4A antibody A18662 available?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-RFPL4A antibody A18662 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-10
Question
We are currently using anti-RFPL4A antibody A18662 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
The anti-RFPL4A antibody (A18662) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-03
Question
Will anti-RFPL4A antibody A18662 work for WB with right lobe of liver?
O. Lewis
Verified customer
Asked: 2018-09-11
Answer
According to the expression profile of right lobe of liver, RFPL4A is highly expressed in right lobe of liver. So, it is likely that anti-RFPL4A antibody A18662 will work for WB with right lobe of liver.
Boster Scientific Support
Answered: 2018-09-11
Question
I was wanting to use your anti-RFPL4A antibody for WB for human right lobe of liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human right lobe of liver identification?
M. Edwards
Verified customer
Asked: 2016-07-29
Answer
As indicated on the product datasheet, A18662 anti-RFPL4A antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right lobe of liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-07-29
Question
I see that the anti-RFPL4A antibody A18662 works with WB, what is the protocol used to produce the result images on the product page?
C. Gonzalez
Verified customer
Asked: 2014-10-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-10-17
