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Product Info Summary
| SKU: | A10349-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-TARC/CCL17/TAAR6 Antibody Picoband®
SKU/Catalog Number
A10349-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TARC/CCL17/TAAR6 Antibody Picoband® catalog # A10349-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TARC/CCL17/TAAR6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10349-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TARC/CCL17/TAAR6, which shares 93.3% and 92.9% amino acid (aa) sequence identity with mouse and rat TAAR6, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10349-1 is reactive to TAAR6 in Human, Monkey, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
41409 MW
Background of TRAR4
Trace amine associated receptor 6, also known as TAAR6, is a protein which in humans is encoded by the TAAR6 gene. This gene encodes a seven-transmembrane G-protein-coupled receptor that likely functions as a receptor for endogenous trace amines. Mutations in this gene may be associated with schizophrenia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10349-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human 293T whole cell, monkey COS-7 whole cell, human U-87MG whole cell, human U251 whole cell, rat brain tissue, mouse brain tissue
FCM: HEL cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TARC/CCL17/TAAR6 using anti-TARC/CCL17/TAAR6 antibody (A10349-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: human U-87MG whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TARC/CCL17/TAAR6 antigen affinity purified polyclonal antibody (Catalog # A10349-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TARC/CCL17/TAAR6 at approximately 38 kDa. The expected band size for TARC/CCL17/TAAR6 is at 38 kDa.
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Figure 2. Flow Cytometry analysis of HEL cells using anti-TARC/CCL17/TAAR6 antibody (A10349-1).
Overlay histogram showing HEL cells stained with A10349-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TARC/CCL17/TAAR6 Antibody (A10349-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For TAAR6 (Source: Uniprot.org, NCBI)
Gene Name
TAAR6
Full Name
Trace amine-associated receptor 6
Weight
41409 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAAR6, check out the TAAR6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAAR6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TARC/CCL17/TAAR6 Antibody Picoband® (A10349-1)
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