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Product Info Summary
| SKU: | M05049-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Synaptophysin SYP Rabbit Monoclonal Antibody
View all Synaptophysin Antibodies
SKU/Catalog Number
M05049-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Synaptophysin SYP Rabbit Monoclonal Antibody catalog # M05049-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Synaptophysin SYP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05049-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BGB-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Synaptophysin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05049-1 is reactive to SYP in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
35 kDa
Calculated molecular weight
33845 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05049-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: SH-SY5Y cell
IHC: mouse brain tissue
ICC/IF: PC-12 cell
Validation Images & Assay Conditions
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Western blot analysis of Synaptophysin expression in SH-SY5Y cell lysate.
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Immunohistochemical analysis of paraffin-embedded mouse brain, using Synaptophysin Antibody .
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Immunofluorescent analysis of PC-12 cells, using Synaptophysin Antibody .
Protein Target Info & Infographic
Gene/Protein Information For SYP (Source: Uniprot.org, NCBI)
Gene Name
SYP
Full Name
Synaptophysin
Weight
33845 MW
Superfamily
synaptophysin/synaptobrevin family
Alternative Names
Synaptophysin;Major synaptic vesicle protein p38;SYP; SYP MRX96, MRXSYP synaptophysin synaptophysin|major synaptic vesicle protein P38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYP, check out the SYP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Synaptophysin SYP Rabbit Monoclonal Antibody (M05049-1)
Hello CJ!
M05049-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lin J,Hao C,Gong Y,Zhang Y,Li Y,Feng Z,Xu X,Huang H,Liao W. Effect of Tetramethylpyrazine on Neuroplasticity after Transient Focal Cerebral Ischemia Reperfusion in Rats. Evid Based Complement Alternat Med. 2021 Jan 18;2021:1587241.doi:10.1155/2021/1587241
Species: Rat
M05049-1 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Liu Y, Liang Z, Liu J, Zou W, Li X, Wang Y, An L. Neural Regen Res. 2013 Oct 15;8(29):2725-33. Doi: 10.3969/J.Issn.1673-5374.2013.29.004. Downregulation Of Caveolin-1 Contributes To The Synaptic Plasticity Deficit In The Hippocampus Of Aged Rats.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Synaptophysin SYP Rabbit Monoclonal Antibody
Question
We have been able to see staining in mouse brain. Do you have any suggestions? Is anti-Synaptophysin Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
According to literature brain does express SYP. According to Uniprot.org, SYP is expressed in anterior cingulate cortex, amygdala, brain, among other tissues. Regarding which tissues have SYP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-06
Question
Do you have a BSA free version of anti-Synaptophysin Rabbit Monoclonal antibody M05049-1 available?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Synaptophysin Rabbit Monoclonal antibody M05049-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-03
Question
I have a question about product M05049-1, anti-Synaptophysin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05049-1 anti-Synaptophysin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-18
Question
I see that the anti-Synaptophysin Rabbit Monoclonal antibody M05049-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-04
Question
Will anti-Synaptophysin Rabbit Monoclonal antibody M05049-1 work for IF with anterior cingulate cortex?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
According to the expression profile of anterior cingulate cortex, SYP is highly expressed in anterior cingulate cortex. So, it is likely that anti-Synaptophysin Rabbit Monoclonal antibody M05049-1 will work for IF with anterior cingulate cortex.
Boster Scientific Support
Answered: 2019-09-09
Question
I was wanting to use your anti-Synaptophysin Rabbit Monoclonal antibody for IF for mouse anterior cingulate cortex on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse anterior cingulate cortex identification?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
You can see on the product datasheet, M05049-1 anti-Synaptophysin Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-22
Question
I was wanting to use using your anti-Synaptophysin Rabbit Monoclonal antibody for regulation of short-term neuronal synaptic plasticity studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Thank you for your inquiry. This M05049-1 anti-Synaptophysin Rabbit Monoclonal antibody is validated on mouse brain. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-20
Question
Is this M05049-1 anti-Synaptophysin Rabbit Monoclonal antibody reactive to the isotypes of SYP?
R. Roberts
Verified customer
Asked: 2019-07-15
Answer
The immunogen of M05049-1 anti-Synaptophysin Rabbit Monoclonal antibody is A synthesized peptide derived from human Synaptophysin . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-15
Question
I would like to test anti-Synaptophysin Rabbit Monoclonal antibody M05049-1 on mouse anterior cingulate cortex for research purposes, then I may be interested in using anti-Synaptophysin Rabbit Monoclonal antibody M05049-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
The products we sell, including anti-Synaptophysin Rabbit Monoclonal antibody M05049-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-09
Question
See attached the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Synaptophysin Rabbit Monoclonal antibody M05049-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-05
Question
Does M05049-1 anti-Synaptophysin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
You can see on the product datasheet, M05049-1 anti-Synaptophysin Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-13
Question
Is a blocking peptide available for product anti-Synaptophysin Rabbit Monoclonal antibody (M05049-1)?
T. Dhar
Verified customer
Asked: 2018-10-19
Answer
We do provide the blocking peptide for product anti-Synaptophysin Rabbit Monoclonal antibody (M05049-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-19
Question
We are currently using anti-Synaptophysin Rabbit Monoclonal antibody M05049-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
The anti-Synaptophysin Rabbit Monoclonal antibody (M05049-1) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Synaptophysin Rabbit Monoclonal antibody M05049-1. Let me know if you need anything else.
E. Zhang
Verified customer
Asked: 2017-09-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-26
Question
My colleagues were happy with the WB result of your anti-Synaptophysin Rabbit Monoclonal antibody. However we have seen positive staining in brain secretory vesicle, using this antibody. Is that expected? Could you tell me where is SYP supposed to be expressed?
Z. Carter
Verified customer
Asked: 2015-07-27
Answer
According to literature, brain does express SYP. Generally SYP expresses in cytoplasmic vesicle, secretory vesicle,. Regarding which tissues have SYP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-07-27
Question
Our lab used your anti-Synaptophysin Rabbit Monoclonal antibody for WB on amygdala in a previous experiment. I am using human, and We are going to use the antibody for IF next. We need examining amygdala as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
M. Zhang
Verified customer
Asked: 2015-02-20
Answer
I have checked the website and datasheets of our anti-Synaptophysin Rabbit Monoclonal antibody and it appears that M05049-1 has been validated on human in both WB and IF. Thus M05049-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-02-20
