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Product Info Summary
| SKU: | M05049-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Synaptophysin SYP Rabbit Monoclonal Antibody, Clone#RM258
View all Synaptophysin Antibodies
SKU/Catalog Number
M05049-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Synaptophysin SYP Rabbit Monoclonal Antibody, Clone#RM258 (Catalog # M05049-2). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Synaptophysin SYP Rabbit Monoclonal Antibody, Clone#RM258 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05049-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM258
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of Synaptophysin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Synaptophysin.
Reactive Species
M05049-2 is reactive to SYP in Human
Applications
M05049-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
33845 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000 dilution
Immunohistochemistry (IHC): 1:200- 1:500 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot analysis of HeLa whole cell lysates, using anti-Synaptophysin rabbit monoclonal antibody (Clone RM258), showed Synaptophysin (~38 kDa) expression in HeLa cells.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue sections using anti-Synaptophysin rabbit monoclonal antibody (Clone RM258) at a 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SYP (Source: Uniprot.org, NCBI)
Gene Name
SYP
Full Name
Synaptophysin
Weight
33845 MW
Superfamily
synaptophysin/synaptobrevin family
Alternative Names
Major synaptic vesicle protein p38; MRX; MRXSYP; Synaptophysin; SYP SYP MRX96, MRXSYP synaptophysin synaptophysin|major synaptic vesicle protein P38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYP, check out the SYP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Synaptophysin SYP Rabbit Monoclonal Antibody, Clone#RM258 (M05049-2)
Hello CJ!
M05049-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu Y, Liang Z, Liu J, Zou W, Li X, Wang Y, An L. Neural Regen Res. 2013 Oct 15;8(29):2725-33. Doi: 10.3969/J.Issn.1673-5374.2013.29.004. Downregulation Of Caveolin-1 Contributes To The Synaptic Plasticity Deficit In The Hippocampus Of Aged Rats.
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Synaptophysin SYP Rabbit Monoclonal Antibody, Clone#RM258
Question
Is a blocking peptide available for product anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 (M05049-2)?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
We do provide the blocking peptide for product anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 (M05049-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-17
Question
Please see the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-27
Question
Does M05049-2 anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
It shows on the product datasheet, M05049-2 anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-15
Question
Does anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2 work for IHC with anterior cingulate cortex?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
According to the expression profile of anterior cingulate cortex, SYP is highly expressed in anterior cingulate cortex. So, it is likely that anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2 will work for IHC with anterior cingulate cortex.
Boster Scientific Support
Answered: 2019-08-13
Question
Our team were well pleased with the WB result of your anti-Synaptophysin Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Based on literature, anterior cingulate cortex does express SYP. Generally SYP expresses in cytoplasmic vesicle, secretory vesicle,. Regarding which tissues have SYP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-16
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-19
Question
Our lab want to know about to test anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2 on human anterior cingulate cortex for research purposes, then I may be interested in using anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Krishna
Verified customer
Asked: 2018-11-16
Answer
The products we sell, including anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-16
Question
Is this M05049-2 anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 reactive to the isotypes of SYP?
Verified Customer
Verified customer
Asked: 2018-11-02
Answer
The immunogen of M05049-2 anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 is A peptide corresponding to the C-terminus of Synaptophysin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-02
Question
I was wanting to use your anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 for IHC for human anterior cingulate cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human anterior cingulate cortex identification?
E. Collins
Verified customer
Asked: 2018-05-29
Answer
You can see on the product datasheet, M05049-2 anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-29
Question
We are currently using anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
The anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 (M05049-2) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-03
Question
We purchased anti-Synaptophysin Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
I looked at the website and datasheets of our anti-Synaptophysin Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2018-01-01
Question
I see that the anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2 works with IHC, what is the protocol used to produce the result images on the product page?
T. Williams
Verified customer
Asked: 2017-03-02
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-03-02
Question
We have observed staining in human amygdala. Do you have any suggestions? Is anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 supposed to stain amygdala positively?
B. Huang
Verified customer
Asked: 2016-06-29
Answer
Based on literature amygdala does express SYP. Based on Uniprot.org, SYP is expressed in anterior cingulate cortex, amygdala, brain, among other tissues. Regarding which tissues have SYP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-06-29
Question
Can you help my question with product M05049-2, anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Patel
Verified customer
Asked: 2016-02-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05049-2 anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-02-25
Question
Do you have a BSA free version of anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2 available?
E. Thomas
Verified customer
Asked: 2014-11-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 M05049-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-11-19
Question
We want using your anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 for regulation of short-term neuronal synaptic plasticity studies. Has this antibody been tested with western blotting on human colon tissue? We would like to see some validation images before ordering.
O. Jones
Verified customer
Asked: 2014-04-08
Answer
Thanks for your inquiry. This M05049-2 anti-Synaptophysin Rabbit Monoclonal antibody, Clone#RM258 is tested on hela whole cell lysates, human colon tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-04-08
