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Product Info Summary
| SKU: | PB9409 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Synaptophysin/SYP Antibody Picoband®
View all Synaptophysin Antibodies
SKU/Catalog Number
PB9409
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Synaptophysin/SYP Antibody Picoband® catalog # PB9409. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Synaptophysin/SYP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9409)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9409 is reactive to SYP in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
33845 MW
Background of Synaptophysin
SYP (Synaptophysin) is located on the short arm of X chromosome (Xp11.23-p11.22). The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present inneuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses. Also, the protein binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9409 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Synaptophysin using anti-Synaptophysin antibody (PB9409).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptophysin antigen affinity purified polyclonal antibody (Catalog # PB9409) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptophysin at approximately 38KD. The expected band size for Synaptophysin is at 34KD.
Protein Target Info & Infographic
Gene/Protein Information For SYP (Source: Uniprot.org, NCBI)
Gene Name
SYP
Full Name
Synaptophysin
Weight
33845 MW
Superfamily
synaptophysin/synaptobrevin family
Alternative Names
Synaptophysin;Major synaptic vesicle protein p38;SYP; SYP MRX96, MRXSYP synaptophysin synaptophysin|major synaptic vesicle protein P38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYP, check out the SYP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Synaptophysin/SYP Antibody Picoband® (PB9409)
Hello CJ!
PB9409 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li Xh, Xin X, Wang Y, Wu Jz, Jin Zd, Ma Ln, Nie Cj, Xiao X, Hu Y, Jin Mw. J Alzheimers Dis. 2013;34(3):755-67. Doi: 10.3233/Jad-122017. Pentamethylquercetin Protects Against Diabetes-Related Cognitive Deficits In Diabetic Goto-Kakizaki Rats.
Liu Y, Liang Z, Liu J, Zou W, Li X, Wang Y, An L. Neural Regen Res. 2013 Oct 15;8(29):2725-33. Doi: 10.3969/J.Issn.1673-5374.2013.29.004. Downregulation Of Caveolin-1 Contributes To The Synaptic Plasticity Deficit In The Hippocampus Of Aged Rats.
Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu Dh, Deng W, Lai Y, Zhou Jh, Zhang Sx, Lin Ww, Chen Df. Brain Res. 2014 Mar 13;1552:41-54. Doi: 10.1016/J.Brainres.2014.01.005. Epub 2014 Jan 20. ??-Asarone Prevents Auto...
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Customer Q&As
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15 Customer Q&As for Anti-Synaptophysin/SYP Antibody Picoband®
Question
I would like using your anti-Synaptophysin/SYP antibody for synaptic vesicle membrane organization studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
We appreciate your inquiry. This PB9409 anti-Synaptophysin/SYP antibody is tested on rat brain tissue, mouse brain. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-09
Question
Is this PB9409 anti-Synaptophysin/SYP antibody reactive to the isotypes of SYP?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
The immunogen of PB9409 anti-Synaptophysin/SYP antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin(1-26aa MLLLADMDVVNQLVAGGQFRVVKEPL), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-05
Question
I am interested in to test anti-Synaptophysin/SYP antibody PB9409 on mouse anterior cingulate cortex for research purposes, then I may be interested in using anti-Synaptophysin/SYP antibody PB9409 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
The products we sell, including anti-Synaptophysin/SYP antibody PB9409, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-07
Question
I have a question about product PB9409, anti-Synaptophysin/SYP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9409 anti-Synaptophysin/SYP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-13
Question
Will PB9409 anti-Synaptophysin/SYP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Krishna
Verified customer
Asked: 2019-09-11
Answer
It shows on the product datasheet, PB9409 anti-Synaptophysin/SYP antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-11
Question
I see that the anti-Synaptophysin/SYP antibody PB9409 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-24
Question
Is there a BSA free version of anti-Synaptophysin/SYP antibody PB9409 available?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Synaptophysin/SYP antibody PB9409 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-03
Question
I was wanting to use your anti-Synaptophysin/SYP antibody for WB for mouse anterior cingulate cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse anterior cingulate cortex identification?
E. Zhang
Verified customer
Asked: 2017-12-18
Answer
It shows on the product datasheet, PB9409 anti-Synaptophysin/SYP antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse anterior cingulate cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-18
Question
Is a blocking peptide available for product anti-Synaptophysin/SYP antibody (PB9409)?
Verified Customer
Verified customer
Asked: 2017-11-22
Answer
We do provide the blocking peptide for product anti-Synaptophysin/SYP antibody (PB9409). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-22
Question
We are currently using anti-Synaptophysin/SYP antibody PB9409 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-31
Answer
The anti-Synaptophysin/SYP antibody (PB9409) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-31
Question
Please see the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Synaptophysin/SYP antibody PB9409. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-07
Question
We have observed staining in rat anterior cingulate cortex. Any tips? Is anti-Synaptophysin/SYP antibody supposed to stain anterior cingulate cortex positively?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
From what I have seen in literature anterior cingulate cortex does express SYP. From what I have seen in Uniprot.org, SYP is expressed in anterior cingulate cortex, amygdala, brain, among other tissues. Regarding which tissues have SYP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-07-20
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for anterior cingulate cortex using anti-Synaptophysin/SYP antibody PB9409. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-05
Question
Would anti-Synaptophysin/SYP antibody PB9409 work for WB with anterior cingulate cortex?
M. Thomas
Verified customer
Asked: 2013-09-17
Answer
According to the expression profile of anterior cingulate cortex, SYP is highly expressed in anterior cingulate cortex. So, it is likely that anti-Synaptophysin/SYP antibody PB9409 will work for WB with anterior cingulate cortex.
Boster Scientific Support
Answered: 2013-09-17
Question
We were happy with the WB result of your anti-Synaptophysin/SYP antibody. However we have observed positive staining in brain cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is SYP supposed to be expressed?
Z. Carter
Verified customer
Asked: 2013-06-21
Answer
Based on literature, brain does express SYP. Generally SYP expresses in cytoplasmic vesicle, secretory vesicle,. Regarding which tissues have SYP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-06-21
