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Product Info Summary
| SKU: | A14692-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Synaptogyrin 3/SYNGR3 Antibody Picoband®
View all Synaptogyrin 3 Antibodies
SKU/Catalog Number
A14692-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Synaptogyrin 3/SYNGR3 Antibody Picoband® catalog # A14692-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Synaptogyrin 3/SYNGR3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14692-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Synaptogyrin 3/SYNGR3 recombinant protein (Position: N45-Y229).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A14692-2 is reactive to SYNGR3 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
64099 MW
Background of Synaptogyrin 3
Synaptogyrin 3 is a protein that in humans is encoded by the SYNGR3 gene. This gene encodes an integral membrane protein. The exact function of this protein is unclear, but studies of a similar murine protein suggest that it is a synaptic vesicle protein that also interacts with the dopamine transporter. The gene product belongs to the synaptogyrin gene family.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14692-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
IHC: mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Synaptogyrin 3/SYNGR3 using anti-Synaptogyrin 3/SYNGR3 antibody (A14692-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptogyrin 3/SYNGR3 antigen affinity purified polyclonal antibody (Catalog # A14692-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptogyrin 3/SYNGR3 at approximately 24 kDa. The expected band size for Synaptogyrin 3/SYNGR3 is at 25 kDa.
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Figure 2. IHC analysis of Synaptogyrin 3/SYNGR3 using anti-Synaptogyrin 3/SYNGR3 antibody (A14692-2).
Synaptogyrin 3/SYNGR3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synaptogyrin 3/SYNGR3 Antibody (A14692-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of Synaptogyrin 3/SYNGR3 using anti-Synaptogyrin 3/SYNGR3 antibody (A14692-2).
Synaptogyrin 3/SYNGR3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synaptogyrin 3/SYNGR3 Antibody (A14692-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SYNGR3 (Source: Uniprot.org, NCBI)
Gene Name
SYNGR3
Full Name
Synaptogyrin-3
Weight
64099 MW
Superfamily
synaptogyrin family
Alternative Names
Transmembrane protein 199; TMEM199; C17orf32 SYNGR3 synaptogyrin 3 synaptogyrin-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYNGR3, check out the SYNGR3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYNGR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Synaptogyrin 3/SYNGR3 Antibody Picoband® (A14692-2)
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