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Product Info Summary
| SKU: | A13929 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SWAN/RBM12 Antibody Picoband®
SKU/Catalog Number
A13929
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SWAN/RBM12 Antibody Picoband® catalog # A13929. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SWAN/RBM12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13929)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SWAN/RBM12 recombinant protein (Position: Q287-E909).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A13929 is reactive to RBM12 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
97 kDa
Calculated molecular weight
64099 MW
Background of RBM12
RNA-binding protein 12 is a protein that in humans is encoded by the RBM12 gene. This gene encodes a protein that contains several RNA-binding motifs, potential transmembrane domains, and proline-rich regions. This gene and the gene for copine I overlap at map location 20q11.21. Alternative splicing in the 5' UTR results in four transcript variants. All variants encode the same protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13929 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human Hela whole cell, human MOLT-4 whole cell, human Daudi whole cell, rat brain tissue, mouse thymus tissue, mouse brain tissue
FCM: RT4 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SWAN/RBM12 using anti-SWAN/RBM12 antibody (A13929).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse thymus tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SWAN/RBM12 antigen affinity purified polyclonal antibody (Catalog # A13929) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SWAN/RBM12 at approximately 97 kDa. The expected band size for SWAN/RBM12 is at 97 kDa.
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Figure 2. Flow Cytometry analysis of RT4 cells using anti-SWAN/RBM12 antibody (A13929).
Overlay histogram showing RT4 cells stained with A13929 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SWAN/RBM12 Antibody (A13929, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RBM12 (Source: Uniprot.org, NCBI)
Gene Name
RBM12
Full Name
RNA-binding protein 12
Weight
64099 MW
Alternative Names
RNA-binding protein 47;RNA-binding motif protein 47;RBM47; RBM12 HRIHFB2091, SCZD19, SWAN RNA binding motif protein 12 RNA-binding protein 12|SH3/WW domain anchor protein in the nucleus
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBM12, check out the RBM12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBM12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SWAN/RBM12 Antibody Picoband® (A13929)
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