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Product Info Summary
| SKU: | M00379-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Survivin Birc5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00379-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Survivin Birc5 Rabbit Monoclonal Antibody catalog # M00379-2. Tested in WB, IHC, IP applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Survivin Birc5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00379-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DGD-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Survivin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00379-2 is reactive to Birc5 in Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
125 kDa
Calculated molecular weight
16298 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00379-2 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:30
Positive Control
WB: Neuro-2a cell
IHC: mouse colon tissue, mouse testis tissue, mouse ovarian cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Survivin expression in Neuro-2a cell lysate (M00379-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Birc5 monoclonal antibody (Catalog # M00379-2) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Birc5
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Survivin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Survivin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse ovarian cancer, using Survivin Antibody.
Protein Target Info & Infographic
Gene/Protein Information For Birc5 (Source: Uniprot.org, NCBI)
Gene Name
Birc5
Full Name
Baculoviral IAP repeat-containing protein 5
Weight
16298 MW
Superfamily
IAP family
Alternative Names
Baculoviral IAP repeat-containing protein 5;Apoptosis inhibitor 4;Apoptosis inhibitor survivin;TIAP;Birc5;Api4, Iap4; BIRC5 API4, EPR-1 baculoviral IAP repeat containing 5 baculoviral IAP repeat-containing protein 5|apoptosis inhibitor 4|apoptosis inhibitor survivin|survivin variant 3 alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Birc5, check out the Birc5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Birc5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Survivin Birc5 Rabbit Monoclonal Antibody (M00379-2)
Hello CJ!
M00379-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...
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Customer Q&As
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15 Customer Q&As for Anti-Survivin Birc5 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Survivin Rabbit Monoclonal antibody (M00379-2)?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
We do provide the blocking peptide for product anti-Survivin Rabbit Monoclonal antibody (M00379-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for myeloid leukemia cell using anti-Survivin Rabbit Monoclonal antibody M00379-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-30
Question
I have a question about product M00379-2, anti-Survivin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00379-2 anti-Survivin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-20
Question
We have observed staining in mouse mammary cancer. Are there any suggestions? Is anti-Survivin Rabbit Monoclonal antibody supposed to stain mammary cancer positively?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Based on literature mammary cancer does express BIRC5. Based on Uniprot.org, BIRC5 is expressed in vagina, myeloid leukemia cell, mammary cancer, neuroblastoma, lung, mammary gland muscle, cervix carcinoma, among other tissues. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722
Boster Scientific Support
Answered: 2019-12-16
Question
We want to test anti-Survivin Rabbit Monoclonal antibody M00379-2 on mouse myeloid leukemia cell for research purposes, then I may be interested in using anti-Survivin Rabbit Monoclonal antibody M00379-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
The products we sell, including anti-Survivin Rabbit Monoclonal antibody M00379-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-27
Question
My boss were happy with the WB result of your anti-Survivin Rabbit Monoclonal antibody. However we have been able to see positive staining in mammary cancer cytoplasm using this antibody. Is that expected? Could you tell me where is BIRC5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Based on literature, mammary cancer does express BIRC5. Generally BIRC5 expresses in cytoplasm. Regarding which tissues have BIRC5 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Lung, Mammary gland, and Muscle, Pubmed ID: 15489334
Mammary cancer, Pubmed ID: 16329164
Myeloid leukemia cell, Pubmed ID: 14702039, 14741722
Boster Scientific Support
Answered: 2019-10-10
Question
We are currently using anti-Survivin Rabbit Monoclonal antibody M00379-2 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
The anti-Survivin Rabbit Monoclonal antibody (M00379-2) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-23
Question
Is this M00379-2 anti-Survivin Rabbit Monoclonal antibody reactive to the isotypes of BIRC5?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The immunogen of M00379-2 anti-Survivin Rabbit Monoclonal antibody is A synthesized peptide derived from human Survivin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-09
Question
Is there a BSA free version of anti-Survivin Rabbit Monoclonal antibody M00379-2 available?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Survivin Rabbit Monoclonal antibody M00379-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-14
Question
Does M00379-2 anti-Survivin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
As indicated on the product datasheet, M00379-2 anti-Survivin Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-06
Question
Here is the WB image, lot number and protocol we used for myeloid leukemia cell using anti-Survivin Rabbit Monoclonal antibody M00379-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-23
Question
I see that the anti-Survivin Rabbit Monoclonal antibody M00379-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-18
Question
We ordered your anti-Survivin Rabbit Monoclonal antibody for IP on mammary cancer last year. I am using rat, and I plan to use the antibody for WB next. We are interested in examining mammary cancer as well as neuroblastoma in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
I have checked the website and datasheets of our anti-Survivin Rabbit Monoclonal antibody and it seems that M00379-2 has been validated on rat in both IP and WB. Thus M00379-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-12-29
Question
Will anti-Survivin Rabbit Monoclonal antibody M00379-2 work for WB with myeloid leukemia cell?
P. Williams
Verified customer
Asked: 2015-07-17
Answer
According to the expression profile of myeloid leukemia cell, BIRC5 is highly expressed in myeloid leukemia cell. So, it is likely that anti-Survivin Rabbit Monoclonal antibody M00379-2 will work for WB with myeloid leukemia cell.
Boster Scientific Support
Answered: 2015-07-17
Question
I was wanting to use your anti-Survivin Rabbit Monoclonal antibody for WB for mouse myeloid leukemia cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse myeloid leukemia cell identification?
A. Evans
Verified customer
Asked: 2013-06-05
Answer
As indicated on the product datasheet, M00379-2 anti-Survivin Rabbit Monoclonal antibody has been validated for IP, IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse myeloid leukemia cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-06-05
