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Product Info Summary
| SKU: | M00238-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Superoxide Dismutase 1 (SOD1) Mouse Monoclonal Antibody [Clone ID: OTI8B10]
View all SOD1/Cu-Zn SOD Antibodies
SKU/Catalog Number
M00238-1
Size
100 µl
Description
Boster Bio SOD1 (Superoxide Dismutase 1) mouse monoclonal antibody, clone OTI8B10 (formerly 8B10). Catalog# M00238-1. Tested in FC, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Superoxide Dismutase 1 (SOD1) Mouse Monoclonal Antibody [Clone ID: OTI8B10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00238-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI8B10
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human SOD1 (NP_000445) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00238-1 is reactive to SOD1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
16-18 kDa
Calculated molecular weight
15.936kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00238-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000~2000
IHC 1:50
FLOW 1:100
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SOD1 using anti-SOD1 antibody (M00238-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SOD1 antigen affinity purified monoclonal antibody (Catalog # M00238-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SOD1 at approximately 16-18 kDa. The expected band size for SOD1 is at 16 kDa.
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HEK293T cells transfected with either SOD1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SOD1 antibody (M00238-1)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOD1.
Protein Target Info & Infographic
Gene/Protein Information For SOD1 (Source: Uniprot.org, NCBI)
Gene Name
SOD1
Full Name
Superoxide dismutase [Cu-Zn]
Weight
15.936kDa
Superfamily
Cu-Zn superoxide dismutase family
Alternative Names
ALS; ALS1; amyotrophic lateral sclerosis 1 (adult); Cu; Cu/Zn superoxide dismutase; CuZn SOD; Cu-Zn SOD; EC 1.15.1.1; homodimer; hSod1; indophenoloxidase A; Ipo1; IPOA; SOD; SOD, cytosolic; SOD, Soluble; SOD1; superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; superoxide dismutase 1, soluble; Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic SOD1 ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer superoxide dismutase 1 superoxide dismutase [Cu-Zn]|Cu/Zn superoxide dismutase|SOD, soluble|epididymis secretory protein Li 44|indophenoloxidase A|superoxide dismutase 1, soluble|superoxide dismutase, cystolic
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOD1, check out the SOD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Superoxide Dismutase 1 (SOD1) Mouse Monoclonal Antibody [Clone ID: OTI8B10] (M00238-1)
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