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Product Info Summary
| SKU: | A01282-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SUMO2/3 Antibody
SKU/Catalog Number
A01282-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SUMO2/3 Antibody (Catalog # A01282-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SUMO2/3 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SUMO2/3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01282-1)
Host
Rabbit
Contents
SUMO2/3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SUMO2/3 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SUMO2. The immunogen is located within the last 50 amino acids of SUMO2/3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Biotin-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.
Reactive Species
A01282-1 is reactive to SUMO2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
10871 MW
Background of Sumo2
Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (Sumo-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, which is a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. All SUMO proteins localize to the nucleus and are covalently conjugated, affecting protein structure, function and interactions. SUMO2 and 3 are 96% identical and are more mobile within nucleus relative to SUMO1. Specific functional differences between SUMO1 and SUMO2 and 3 remain to be identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01282-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SUMO2/3 antibody can be used for detection of SUMO2 and SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SUMO2/3 in Jurkat cell lysate with SUMO2/3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 5 μg/mL.
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Immunofluorescence of SUMO2/3 in rat liver tissue with SUMO2/3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SUMO2 (Source: Uniprot.org, NCBI)
Gene Name
SUMO2
Full Name
Small ubiquitin-related modifier 2
Weight
10871 MW
Superfamily
ubiquitin family
Alternative Names
HSMT3, SMT3B, SUMO3, Smt3A, SMT3H2, SMT3A, Small ubiquitin-related modifier 2, HSMT3, SUMO-2 SUMO2 HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A small ubiquitin like modifier 2 small ubiquitin-related modifier 2|SMT3 homolog 2|SMT3 suppressor of mif two 3 homolog 2|sentrin 2|ubiquitin-like protein SMT3A|ubiquitin-like protein SMT3B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SUMO2, check out the SUMO2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SUMO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SUMO2/3 Antibody (A01282-1)
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