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Product Info Summary
| SKU: | A00631 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Sumo SUMO1 Antibody
SKU/Catalog Number
A00631
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Sumo SUMO1 Antibody (Catalog # A00631). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Sumo antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Sumo SUMO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00631)
Host
Rabbit
Contents
Sumo Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Sumo antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human sumo. The immunogen is located within the first 50 amino acids of Sumo.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
CRH antibody is human specific.
Reactive Species
A00631 is reactive to SUMO1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
11557 MW
Background of SUMO1
The sumo family of proteins is related both structurally and functionally to ubiquitin in that they are post-translationally attached to the e-amino group of a lysine residue of the substrate protein. This sumoylation plays a number of roles in DNA replication and repair, protein targeting to various subnuclear structures, and the regulation of numerous cellular processes including the inflammatory response in mammalian cells. Sumo was initially identified as a covalent modification of RanGAP1 in studies on nuclear import in mammalian cells. More recently, sumo has been shown to be involved in the regulation of transcription factors, possibly by enhancing their interactions with co-repressors. Sumo is also thought to play some role in the modulation of ubiquitin-mediated degradation of proteins by acting as an inhibitor. At least four different isoforms of sumo are known to exist; Sumo antibody will only recognize isoform 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00631 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Sumo antibody can be used for detection of sumo by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of sumo in HL-60 cell lysate with sumo antibody at (A) 0.5
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Immunocytochemistry of Sumo in HL60 cells with Sumo antibody at 5 μg/mL.
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Immunofluorescence of Sumo in HL60 cells with Sumo antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SUMO1 (Source: Uniprot.org, NCBI)
Gene Name
SUMO1
Full Name
Small ubiquitin-related modifier 1
Weight
11557 MW
Superfamily
ubiquitin family
Alternative Names
DAP1, GMP1, PIC1, SMT3, UBL1, OFC10, SENP2, SMT3C, SMT3H3, OK/SW-cl.43, Small ubiquitin-related modifier 1, GAP-modifying protein 1, SUMO-1 SUMO1 DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1 small ubiquitin like modifier 1 small ubiquitin-related modifier 1|GAP modifying protein 1|SMT3 homolog 3|SMT3 suppressor of mif two 3 homolog 1|sentrin|ubiquitin-homology domain protein PIC1|ubiquitin-like protein SMT3C|ubiquitin-like protein UBL1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SUMO1, check out the SUMO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SUMO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sumo SUMO1 Antibody (A00631)
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