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Product Info Summary
| SKU: | M00631-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Sumo 1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00631-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Sumo 1 Rabbit Monoclonal Antibody catalog # M00631-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sumo 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00631-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BFI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Sumo 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00631-1 is reactive to SUMO1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
26 kDa
Calculated molecular weight
11557 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00631-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: human breast carcinoma tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of SUMO1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Sumo 1 Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For SUMO1 (Source: Uniprot.org, NCBI)
Gene Name
SUMO1
Full Name
Small ubiquitin-related modifier 1
Weight
11557 MW
Superfamily
ubiquitin family
Alternative Names
Small ubiquitin-related modifier 1;SUMO-1;GAP-modifying protein 1;GMP1;SMT3 homolog 3;Sentrin;Ubiquitin-homology domain protein PIC1;Ubiquitin-like protein SMT3C;Smt3C;Ubiquitin-like protein UBL1;SUMO1;SMT3C, SMT3H3, UBL1;OK/SW-cl.43; SUMO1 DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1 small ubiquitin like modifier 1 small ubiquitin-related modifier 1|GAP modifying protein 1|SMT3 homolog 3|SMT3 suppressor of mif two 3 homolog 1|sentrin|ubiquitin-homology domain protein PIC1|ubiquitin-like protein SMT3C|ubiquitin-like protein UBL1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SUMO1, check out the SUMO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SUMO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sumo 1 Rabbit Monoclonal Antibody (M00631-1)
Hello CJ!
M00631-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The SUMO Protease SENP3 Orchestrates G2-M Transition and Spindle Assembly in Mouse Oocytes
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Sumo 1 Rabbit Monoclonal Antibody
Question
We are currently using anti-Sumo 1 Rabbit Monoclonal antibody M00631-1 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The anti-Sumo 1 Rabbit Monoclonal antibody (M00631-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
I was wanting to use your anti-Sumo 1 Rabbit Monoclonal antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
As indicated on the product datasheet, M00631-1 anti-Sumo 1 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-07
Question
We have seen staining in rat brain. What should we do? Is anti-Sumo 1 Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
From what I have seen in literature brain does express SUMO1. From what I have seen in Uniprot.org, SUMO1 is expressed in caudate nucleus, brain, brain placenta, placenta, colon adenocarcinoma, amygdala, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SUMO1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 9119407
Brain, and Placenta, Pubmed ID: 8806687
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-05
Question
Will anti-Sumo 1 Rabbit Monoclonal antibody M00631-1 work for WB with brain?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
According to the expression profile of brain, SUMO1 is highly expressed in brain. So, it is likely that anti-Sumo 1 Rabbit Monoclonal antibody M00631-1 will work for WB with brain.
Boster Scientific Support
Answered: 2020-02-21
Question
Here is the WB image, lot number and protocol we used for brain using anti-Sumo 1 Rabbit Monoclonal antibody M00631-1. Please let me know if you require anything else.
H. Johnson
Verified customer
Asked: 2020-02-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-12
Question
My boss were satisfied with the WB result of your anti-Sumo 1 Rabbit Monoclonal antibody. However we have observed positive staining in brain placenta nucleus membrane. nucleus speckle. using this antibody. Is that expected? Could you tell me where is SUMO1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
From what I have seen in literature, brain placenta does express SUMO1. Generally SUMO1 expresses in nucleus membrane. nucleus speckle. Regarding which tissues have SUMO1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 9119407
Brain, and Placenta, Pubmed ID: 8806687
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231, 20388717
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-12-27
Question
Do you have a BSA free version of anti-Sumo 1 Rabbit Monoclonal antibody M00631-1 available?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Sumo 1 Rabbit Monoclonal antibody M00631-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Sumo 1 Rabbit Monoclonal antibody M00631-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-08
Question
Is a blocking peptide available for product anti-Sumo 1 Rabbit Monoclonal antibody (M00631-1)?
Verified Customer
Verified customer
Asked: 2019-02-14
Answer
We do provide the blocking peptide for product anti-Sumo 1 Rabbit Monoclonal antibody (M00631-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-14
Question
We are interested in using your anti-Sumo 1 Rabbit Monoclonal antibody for cellular response to cadmium ion studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
B. Kulkarni
Verified customer
Asked: 2018-08-17
Answer
Thanks for your inquiry. This M00631-1 anti-Sumo 1 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-17
Question
We have tried in the past anti-Sumo 1 Rabbit Monoclonal antibody for IP on cervix carcinoma last year. I am using mouse, and We want to use the antibody for IF next. I would like examining cervix carcinoma as well as cervix carcinoma erythroleukemia in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-08-01
Answer
I looked at the website and datasheets of our anti-Sumo 1 Rabbit Monoclonal antibody and I see that M00631-1 has been tested on mouse in both IP and IF. Thus M00631-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-08-01
Question
Is this M00631-1 anti-Sumo 1 Rabbit Monoclonal antibody reactive to the isotypes of SUMO1?
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
The immunogen of M00631-1 anti-Sumo 1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Sumo 1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-20
Question
Our lab want to know about to test anti-Sumo 1 Rabbit Monoclonal antibody M00631-1 on rat brain for research purposes, then I may be interested in using anti-Sumo 1 Rabbit Monoclonal antibody M00631-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Zhao
Verified customer
Asked: 2015-10-13
Answer
The products we sell, including anti-Sumo 1 Rabbit Monoclonal antibody M00631-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-10-13
Question
I see that the anti-Sumo 1 Rabbit Monoclonal antibody M00631-1 works with WB, what is the protocol used to produce the result images on the product page?
C. Carter
Verified customer
Asked: 2015-04-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-04-28
Question
Can you help my question with product M00631-1, anti-Sumo 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Evans
Verified customer
Asked: 2015-03-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00631-1 anti-Sumo 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-03-19
Question
Would M00631-1 anti-Sumo 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Carter
Verified customer
Asked: 2013-08-19
Answer
It shows on the product datasheet, M00631-1 anti-Sumo 1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-08-19
