Product Info Summary
SKU: | M02224 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-STK3/Mst2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02224
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-STK3/Mst2 Rabbit Monoclonal Antibody catalog # M02224. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STK3/Mst2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02224)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EIE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human STK3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02224 is reactive to STK3 in Human, Mouse, Rat
Applications
M02224 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
56301 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:50
FC 1:50
Validation Images & Assay Conditions
![m02224 wb m02224 wb](https://www.bosterbio.com/media/catalog/product/m/0/m02224-wb.jpg)
Click image to see more details
Western blot analysis of STK3 expression in HeLa cell lysate.
![m02224 ihc m02224 ihc](https://www.bosterbio.com/media/catalog/product/m/0/m02224-ihc.jpg)
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human kidney, using STK3 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For STK3 (Source: Uniprot.org, NCBI)
Gene Name
STK3
Full Name
Serine/threonine-protein kinase 3
Weight
56301 MW
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; FLJ90748; KRS1; Mammalian STE20-like protein kinase 2; Mess1; MST2; MST-2; MST2EC 2.7.11.1; MST3; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); serine/threonine kinase 3; serine/threonine-protein kinase 3; Serine/threonine-protein kinase Krs-1; STE20-like kinase MST2; STK3 STK3 KRS1, MST2 serine/threonine kinase 3 serine/threonine-protein kinase 3|KB-1458E12.1|MST-2|STE20-like kinase MST2|epididymis secretory sperm binding protein|hippo homolog|mammalian STE20-like protein kinase 2|serine/threonine kinase 3 (STE20 homolog, yeast)|serine/threonine kinase 3 (Ste20, yeast homolog)|serine/threonine-protein kinase Krs-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STK3, check out the STK3 Infographic
![STK3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STK3/Mst2 Rabbit Monoclonal Antibody (M02224)
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No publications found for M02224
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Customer Q&As
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4 Customer Q&As for Anti-STK3/Mst2 Rabbit Monoclonal Antibody
Question
I have a question about product M02224, anti-STK3/Mst2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02224 anti-STK3/Mst2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-31
Question
We are currently using anti-STK3/Mst2 Rabbit Monoclonal antibody M02224 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
The anti-STK3/Mst2 Rabbit Monoclonal antibody (M02224) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-02
Question
See attached the WB image, lot number and protocol we used for uterus using anti-STK3/Mst2 Rabbit Monoclonal antibody M02224. Please let me know if you require anything else.
B. Jones
Verified customer
Asked: 2017-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-02-13
Question
I see that the anti-STK3/Mst2 Rabbit Monoclonal antibody M02224 works with WB, what is the protocol used to produce the result images on the product page?
D. Gonzalez
Verified customer
Asked: 2014-09-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-09-23