Anti-Human CYP1A1 DyLight® 550 conjugated Antibody

Product Name

Anti-Human CYP1A1 DyLight® 550 conjugated Antibody

View all CYP1A1 Antibodies

SKU/Catalog Number

A00471-Dyl550

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human CYP1A1 DyLight® 550 conjugated Antibody catalog # A00471-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human CYP1A1 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00471-Dyl550)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00471-Dyl550 is reactive to CYP1A1 in Human

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

58.165kDa

Background of CYP1A1

CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline). It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo (a)pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7,8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7,8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7,8-dihydrodiol-9,10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo (a)pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo (a)pyrene carcinogen into the systemic circulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00471-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Validation Images & Assay Conditions

Gene/Protein Information For CYP1A1 (Source: Uniprot.org, NCBI)

Alternative Names

Cytochrome P450 1A1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; CYP1A1 CYP1A1 AHH, AHRR, CP11, CYP1, CYPIA1, P1-450, P450-C, P450DX cytochrome P450 family 1 subfamily A member 1 cytochrome P450 1A1|aryl hydrocarbon hydroxylase|cytochrome P1-450, dioxin-inducible|cytochrome P450 form 6|cytochrome P450, family 1, subfamily A, polypeptide 1|cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1|cytochrome P450-C|cytochrome P450-P1|flavoprotein-linked monooxygenase|hydroperoxy icosatetraenoate dehydratase|xenobiotic monooxygenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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