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Product Info Summary
| SKU: | M02947-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Sterol carrier protein 2 Rabbit Monoclonal Antibody
View all Sterol carrier protein 2 Antibodies
SKU/Catalog Number
M02947-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Sterol carrier protein 2 Rabbit Monoclonal Antibody catalog # M02947-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sterol carrier protein 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02947-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
28S05
Isotype
IgG
Immunogen
A synthesized peptide derived from human Sterol carrier protein 2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02947-1 is reactive to SCP2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
59 kDa
Calculated molecular weight
56560 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02947-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human HepG2 whole cell, human HUH-7 whole cell, rat RH35 whole cell, mouse liver tissue, mouse HEPA1-6 whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Sterol carrier protein 2 using anti-Sterol carrier protein 2 antibody (M02947-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HUH-7 whole cell lysates,
Lane 3: rat RH35 whole cell lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sterol carrier protein 2 antigen affinity purified monoclonal antibody (Catalog # M02947-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sterol carrier protein 2 at approximately 59 kDa. The expected band size for Sterol carrier protein 2 is at 59 kDa.
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Figure 2. IF analysis of Sterol carrier protein 2 using anti-Sterol carrier protein 2 antibody (M02947-1).
Sterol carrier protein 2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-Sterol carrier protein 2 Antibody (M02947-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For SCP2 (Source: Uniprot.org, NCBI)
Gene Name
SCP2
Full Name
Non-specific lipid-transfer protein
Weight
56560 MW
Alternative Names
ATP synthase subunit beta, mitochondrial;3.6.3.14;ATP5B;ATPMB, ATPSB; SCP2 NLTP, NSL-TP, SCOX, SCP-2, SCP-CHI, SCP-X, SCPX sterol carrier protein 2 sterol carrier protein 2|SCP-2/3-oxoacyl-CoA thiolase|SCP-2/thiolase|acetyl-CoA C-myristoyltransferase|epididymis secretory sperm binding protein|non-specific lipid-transfer protein|propanoyl-CoA C-acyltransferase|sterol carrier protein X|straight-chain acyl-CoA oxidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCP2, check out the SCP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sterol carrier protein 2 Rabbit Monoclonal Antibody (M02947-1)
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