Anti-Adipophilin / Perilipin-2 (Marker of Obesity) Monoclonal Antibody

Product Name

Anti-Adipophilin / Perilipin-2 (Marker of Obesity) Monoclonal Antibody

View all Perilipin-2/ADFP Antibodies

SKU/Catalog Number

M04328

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Adipophilin / Perilipin-2 (Marker of Obesity) Monoclonal Antibody (Catalog # M04328). Tested in ELISA, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Adipophilin / Perilipin-2 (Marker of Obesity) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04328)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: ADFP/1494

Isotype

IgG2b, lambda

Immunogen

Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M04328 is reactive to PLIN2 in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

48075 MW

Background of Perilipin-2/ADFP

Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.Â

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M04328 is guaranteed for ELISA, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (For coating, order antibody without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For PLIN2 (Source: Uniprot.org, NCBI)

Alternative Names

Perilipin-2;Adipophilin;Adipose differentiation-related protein;ADRP;PLIN2;ADFP; PLIN2 ADFP, ADRP perilipin 2 perilipin-2|adipophilin|adipose differentiation-related protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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