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Product Info Summary
| SKU: | A08410-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-STEA2 Antibody (Center)
SKU/Catalog Number
A08410-2
Size
80 µl
Description
Boster Bio Anti-STEA2 Antibody (Center) (Catalog # A08410-2). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-STEA2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08410-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This STEAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-258 amino acids of human STEAP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08410-2 is reactive to STEAP2 in Human, Mouse
Reconstitution
Calculated molecular weight
56056 Da
Background of Steap2
This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08410-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from 293, HepG2 cell line, mouse spleen tissue lysate (from left to right), using STEAP2 Antibody (Center). A08410-2 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Western blot analysis of extracts from 293 (lanes 1 and 2),HepG2 (lanes 3 and 4)cell line and mouse spleen (lanes 5 and 6)tissue lysate: 1, 3, 5. using STEAP2 Antibody (Center)( A08410-2), (1:500). 2, 4, 6. using STEAP2 Antibody (Center), preincubated with the control peptide antigen.
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STEAP2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the STEAP2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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STEAP2 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For STEAP2 (Source: Uniprot.org, NCBI)
Gene Name
STEAP2
Full Name
Metalloreductase STEAP2
Weight
56056 Da
Superfamily
STEAP family
Alternative Names
Metalloreductase STEAP2, 1161-, Prostate cancer-associated protein 1, Protein up-regulated in metastatic prostate cancer, PUMPCn, Six-transmembrane epithelial antigen of prostate 2, SixTransMembrane protein of prostate 1, STEAP2, PCANAP1, STAMP1 Steap2|4921538B17Rik, AI930049, AW045895, IPCA-1, IPCA1, PCANAP1, STAMP1, STMP|six transmembrane epithelial of prostate 2|metalloreductase STEAP2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STEAP2, check out the STEAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STEAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STEA2 Antibody (Center) (A08410-2)
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