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Product Info Summary
| SKU: | M00036-3 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IP, IHC-P, WB |
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Product info
Product Name
Anti-STAT1 Purified Monoclonal Antibody
SKU/Catalog Number
M00036-3
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-STAT1 Purified Monoclonal Antibody (Catalog# M00036-3). Tested in IP, WB, IHC-P application(s). This antibody reacts with Mouse, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-STAT1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00036-3)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SM1
Isotype
Mouse IgG2b
Immunogen
STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE). The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00036-3 is reactive to STAT1 in Human, Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
87335 MW
Background of STAT1
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00036-3 is guaranteed for IP, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (paraffin sections): 5 μg/ml; positive tissue: lung macrophages.
Western blotting: 1-2 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-STAT1 Purified Monoclonal Antibody M00036-3
Detection of Human phosphorylated STAT1[p Ser727] and total STAT1 by Western Blot. Western blot shows lysate of human malignant melanoma cells (short-term culture derived from a patient) in response to interferon treatment to induce phosphorylation of STAT1 at Ser727. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Specific band was detected for phosphorylated STAT1[Ser727] by the antibody PSM1 (upper panel) and total STAT1 level by the antibody SM1 (lower panel).Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For STAT1 (Source: Uniprot.org, NCBI)
Gene Name
STAT1
Full Name
Signal transducer and activator of transcription 1-alpha/beta
Weight
87335 MW
Superfamily
transcription factor STAT family
Alternative Names
CANDF7, IMD31, ISGF3 STAT1 CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91 signal transducer and activator of transcription 1 signal transducer and activator of transcription 1-alpha/beta|signal transducer and activator of transcription 1, 91kD|signal transducer and activator of transcription 1, 91kDa|transcription factor ISGF-3 components p91/p84
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAT1, check out the STAT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAT1 Purified Monoclonal Antibody (M00036-3)
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