Anti-STAM2 Antibody Picoband™

Product Name

Anti-STAM2 Antibody Picoband™

View all STAM2 Antibodies

SKU/Catalog Number

A06533-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-STAM2 Antibody Picoband™ catalog # A14386-1. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-STAM2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06533-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human STAM2 recombinant protein (Position: K134-L525). Human STAM2 shares 82.7% and 79.6% amino acid (aa) sequence identity with mouse and rat STAM2, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06533-3 is reactive to STAM2 in Human, Mouse, Rat

Applications

A06533-3 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

65-70 kDa

Calculated molecular weight

52588 MW

Background of STAM2

Signal transducing adapter molecule 2 is a protein that in humans is encoded by the STAM2 gene. The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg /1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of STAM2 using anti-STAM2 antibody (A06533-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAM2 antigen affinity purified polyclonal antibody (Catalog # A06533-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAM2 at approximately 65-70 kDa. The expected band size for STAM2 is at 58 kDa.

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Figure 2. IF analysis of STAM2 using anti-STAM2 antibody (A06533-3) and anti-Beta Tubulin antibody (M01857-3).
STAM2 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STAM2 Antibody (A06533-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of Hela cells using anti-STAM2 antibody (A06533-3).
Overlay histogram showing Hela cells stained with A06533-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAM2 Antibody (A06533-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For STAM2 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp564C047; Hbp; Hrs-binding protein; HSE1 homolog; signal transducing adapter molecule 2; signal transducing adaptor molecule (SH3 domain and ITAM motif) 2; STAM-2; STAM2A; STAM2B; STAM-like protein containing SH3 and ITAM domains 2 STAM2 HbpA, STAM2B, STAM2 signal transducing adaptor molecule 2 signal transducing adapter molecule 2|HSE1 homolog|Hrs-binding protein|STAM-2|STAM-like protein containing SH3 and ITAM domains 2|signal transducing adaptor molecule (SH3 domain and ITAM motif) 2

For more info on STAM2, check out the STAM2 Infographic

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In this infographic, you will see the following information for STAM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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