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Product Info Summary
| SKU: | A00864-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-STAM1 Antibody Picoband®
SKU/Catalog Number
A00864-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-STAM1 Antibody Picoband® catalog # A00864-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00864-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human STAM1 recombinant protein (Position: F9-Q254).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00864-1 is reactive to STAM in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
59 kDa
Calculated molecular weight
34632 MW
Background of STAM-1
Signal transducing adapter molecule 1 is a protein that in humans is encoded by the STAM gene. This gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The encoded protein also associates with hepatocyte growth factor-regulated substrate to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00864-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HepG2 whole cell, human PANC-1 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of STAM1 using anti-STAM1 antibody (A00864-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAM1 antigen affinity purified polyclonal antibody (Catalog # A00864-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAM1 at approximately 59KD. The expected band size for STAM1 is at 59KD.
Protein Target Info & Infographic
Gene/Protein Information For STAM (Source: Uniprot.org, NCBI)
Gene Name
STAM
Full Name
Signal transducing adapter molecule 1
Weight
34632 MW
Superfamily
STAM family
Alternative Names
Signal transducing adapter molecule 1; STAM-1; STAM; STAM1 STAM STAM-11, STAM signal transducing adaptor molecule signal transducing adapter molecule 1|HSE1 homolog|signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STAM, check out the STAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-STAM1 Antibody Picoband® (A00864-1)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-STAM1 Antibody Picoband®
Question
Is this A00864-1 anti-STAM1 antibody reactive to the isotypes of STAM?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The immunogen of A00864-1 anti-STAM1 antibody is E. coli-derived human STAM1 recombinant protein (Position: F9-Q254). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-14
Question
I am interested in to test anti-STAM1 antibody A00864-1 on rat liver for research purposes, then I may be interested in using anti-STAM1 antibody A00864-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The products we sell, including anti-STAM1 antibody A00864-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-28
Question
We are currently using anti-STAM1 antibody A00864-1 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
The anti-STAM1 antibody (A00864-1) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-05
