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Product Info Summary
| SKU: | A30497 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-SSXT SS18 Antibody
SKU/Catalog Number
A30497
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SSXT SS18 Antibody (Catalog# A30497). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SSXT SS18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30497)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SSXT. Immunogen sequence location: 1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30497 is reactive to SS18 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
45929 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30497 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HepG2, Jurkat, and COLO205 cells, using SSXT Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For SS18 (Source: Uniprot.org, NCBI)
Gene Name
SS18
Full Name
Protein SSXT
Weight
45929 MW
Superfamily
SS18 family
Alternative Names
SS18;SSXT;SYT;Protein SSXT;Protein SYT;Synovial sarcoma translocated to X chromosome protein SS18 SSXT, SYT SS18 subunit of BAF chromatin remodeling complex protein SSXT|SS18, nBAF chromatin remodeling complex subunit|synovial sarcoma translocated to X chromosome protein|synovial sarcoma translocation, chromosome 18|synovial sarcoma, translocated to X chromosome
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SS18, check out the SS18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SS18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SSXT SS18 Antibody (A30497)
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No publications found for A30497
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-SSXT SS18 Antibody
Question
Does A30497 anti-SSXT antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
As indicated on the product datasheet, A30497 anti-SSXT antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-27
Question
I see that the anti-SSXT antibody A30497 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-25
Question
We are currently using anti-SSXT antibody A30497 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
N. Taylor
Verified customer
Asked: 2015-02-11
Answer
The anti-SSXT antibody (A30497) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-02-11
Question
Do you have a BSA free version of anti-SSXT antibody A30497 available?
F. Krishna
Verified customer
Asked: 2014-07-14
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SSXT antibody A30497 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-07-14
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-SSXT antibody A30497. Let me know if you need anything else.
A. Anderson
Verified customer
Asked: 2014-07-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-04
Question
Is a blocking peptide available for product anti-SSXT antibody (A30497)?
D. Johnson
Verified customer
Asked: 2014-06-17
Answer
We do provide the blocking peptide for product anti-SSXT antibody (A30497). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-06-17
