Click image to see more details
Product Info Summary
| SKU: | A10463-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SR-1F HTR1F Antibody
SKU/Catalog Number
A10463-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SR-1F HTR1F Antibody catalog # A10463-1. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SR-1F HTR1F Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10463-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human 5-HT-1F. AA range:101-150
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10463-1 is reactive to HTR1F in Human, Monkey, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
41709 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10463-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using SR-1F Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For HTR1F (Source: Uniprot.org, NCBI)
Gene Name
HTR1F
Full Name
5-hydroxytryptamine receptor 1F
Weight
41709 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
5-hydroxytryptamine receptor 1F;5-HT-1F;5-HT1F;Serotonin receptor 1F;HTR1F;HTR1EL; HTR1F 5-HT-1F, 5-HT1F, 5HT6, HTR1EL, MR77 5-hydroxytryptamine receptor 1F 5-hydroxytryptamine receptor 1F|5-hydroxytryptamine (serotonin) receptor 1F, G protein-coupled|serotonin receptor 1F
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HTR1F, check out the HTR1F Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HTR1F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SR-1F HTR1F Antibody (A10463-1)
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No publications found for A10463-1
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SR-1F HTR1F Antibody
Question
Is a blocking peptide available for product anti-SR-1F antibody (A10463-1)?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
We do provide the blocking peptide for product anti-SR-1F antibody (A10463-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-02
Question
We are currently using anti-SR-1F antibody A10463-1 for monkey tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The anti-SR-1F antibody (A10463-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-11
Question
I have a question about product A10463-1, anti-SR-1F antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Lewis
Verified customer
Asked: 2019-05-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10463-1 anti-SR-1F antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-SR-1F antibody A10463-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-01
Question
Does A10463-1 anti-SR-1F antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Patel
Verified customer
Asked: 2015-05-18
Answer
It shows on the product datasheet, A10463-1 anti-SR-1F antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-05-18
Question
I was wanting to use your anti-SR-1F antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
B. Miller
Verified customer
Asked: 2014-12-25
Answer
It shows on the product datasheet, A10463-1 anti-SR-1F antibody has been validated for WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-25
Question
Is this A10463-1 anti-SR-1F antibody reactive to the isotypes of HTR1F?
D. Bhatt
Verified customer
Asked: 2013-05-09
Answer
The immunogen of A10463-1 anti-SR-1F antibody is Synthesized peptide derived from the Internal region of human SR-1F. at AA rangle: 70-150. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-05-09
