Anti-SR-1F HTR1F Antibody

5-HT1F antibody

Boster Bio Anti-SR-1F HTR1F Antibody catalog # A10463-1. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Product Info Summary

SKU: A10463-1
Size: 100μl
Reactive Species: Human, Monkey, Mouse, Rat
Host: Rabbit
Application: WB

Customers Who Bought This Also Bought

Product Name

Anti-SR-1F HTR1F Antibody

View all 5-HT1F Antibodies

SKU/Catalog Number

A10463-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-SR-1F HTR1F Antibody catalog # A10463-1. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SR-1F HTR1F Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10463-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human SR-1F. at AA rangle: 70-150

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10463-1 is reactive to HTR1F in Human, Monkey, Mouse, Rat

Applications

A10463-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

41709 MW

Background of 5-HT1F

Cybr (Pscdbp) is a new scaffold protein with significant homology to Tamalin. It contains a PDZ binding domain and a coiled-coil domain. Cybr is highly expressed in the immune system, and its expression seems to be common to nearly all immune cell populations (T cells, B cells, NK Macrophages, dendritic cells).  Other organs/tissues in which Cybr is expressed include lung, kidney, intestine and testes. Cybr may play an important in vivo role in cell migration, especially when modulated by pro-inflammatory cytokines. Anti-Cybr Antibody is suitable for researchers interested in the Research Areas of Chromatin, Immunology, Neuroscience, and Signal Transduction.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For HTR1F (Source: Uniprot.org, NCBI)

Gene Name

HTR1F

Full Name

5-hydroxytryptamine receptor 1F

Weight

41709 MW

Superfamily

G-protein coupled receptor 1 family

Alternative Names

5HT1F; 5-HT1F; 5-HT-1F; 5HT6; 5-hydroxytryptamine (serotonin) receptor 1F; HTR1EL; HTR1ELMR77,5-HT1F5-hydroxytryptamine receptor 1F; HTR1F; MR77; Serotonin receptor 1F HTR1F 5-HT-1F, 5-HT1F, 5HT6, HTR1EL, MR77 5-hydroxytryptamine receptor 1F 5-hydroxytryptamine receptor 1F|5-hydroxytryptamine (serotonin) receptor 1F, G protein-coupled|serotonin receptor 1F

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HTR1F, check out the HTR1F Infographic

HTR1F infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HTR1F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A10463-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-SR-1F HTR1F Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-SR-1F HTR1F Antibody

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-SR-1F HTR1F Antibody

Question

Is a blocking peptide available for product anti-SR-1F antibody (A10463-1)?

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

We do provide the blocking peptide for product anti-SR-1F antibody (A10463-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-02

Question

We are currently using anti-SR-1F antibody A10463-1 for monkey tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

The anti-SR-1F antibody (A10463-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-11

Question

I have a question about product A10463-1, anti-SR-1F antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

C. Lewis

Verified customer

Asked: 2019-05-07

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10463-1 anti-SR-1F antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-07

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-SR-1F antibody A10463-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-06-01

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-01

Question

Does A10463-1 anti-SR-1F antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Patel

Verified customer

Asked: 2015-05-18

Answer

It shows on the product datasheet, A10463-1 anti-SR-1F antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-05-18

Question

I was wanting to use your anti-SR-1F antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?

B. Miller

Verified customer

Asked: 2014-12-25

Answer

It shows on the product datasheet, A10463-1 anti-SR-1F antibody has been validated for WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-12-25

Question

Is this A10463-1 anti-SR-1F antibody reactive to the isotypes of HTR1F?

D. Bhatt

Verified customer

Asked: 2013-05-09

Answer

The immunogen of A10463-1 anti-SR-1F antibody is Synthesized peptide derived from the Internal region of human SR-1F. at AA rangle: 70-150. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-05-09

Order DetailsPrice
A10463-1

100uL

$399

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A10463-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.